Western Blot (WB)
(SMPD3 Antibody (N-term) (MBS9203057) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SMPD3 antibody detected the SMPD3 protein (arrow).)
Rabbit SMPD3 Polyclonal Antibody | anti-SMPD3 antibody
SMPD3 Antibody (N-term)
Gene Names
SMPD3; NSMASE2
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SMPD3; Polyclonal Antibody; SMPD3 Antibody (N-term); Sphingomyelin phosphodiesterase 3; Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II; anti-SMPD3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB39878
Specificity
This SMPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the N-terminal region of human SMPD3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
137-165
Sequence Length
647
Applicable Applications for anti-SMPD3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Cellular Location
Golgi apparatus membrane; Lipid-anchor. Cellmembrane; Lipid-anchor. Note=May localizeto detergent- resistant subdomains of Golgimembranes of hypothalamic neurosecretoryneurons (PubMed:10823942). Localizes toplasma membrane in confluentcontact-inhaibited cells (PubMed:15051724)
Tissue Location
Predominantly expressed in brain
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thawcycles.
Western Blot (WB)
(SMPD3 Antibody (N-term) (MBS9203057) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SMPD3 antibody detected the SMPD3 protein (arrow).)
Western Blot (WB)
(SMPD3 Antibody (N-term) (MBS9203057) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SMPD3 antibody detected the SMPD3 protein (arrow).)
Related Product Information for anti-SMPD3 antibody
SMPD3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
Product Categories/Family for anti-SMPD3 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Filosto, S., et al. J. Biol. Chem. 285(14):10213-10222(2010)
Maupas-Schwalm, F., et al. Cell. Signal. 21(12):1925-1934(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Ito, H., et al. Biochim. Biophys. Acta 1789 (11-12), 681-690 (2009) :
Filosto, S., et al. J. Biol. Chem. 285(14):10213-10222(2010)
Maupas-Schwalm, F., et al. Cell. Signal. 21(12):1925-1934(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Ito, H., et al. Biochim. Biophys. Acta 1789 (11-12), 681-690 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71081
NCBI Official Full Name
sphingomyelin phosphodiesterase 3
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
NCBI Official Symbol
SMPD3
NCBI Official Synonym Symbols
NSMASE2
NCBI Protein Information
sphingomyelin phosphodiesterase 3
UniProt Protein Name
Sphingomyelin phosphodiesterase 3
Protein Family
UniProt Gene Name
SMPD3
UniProt Synonym Gene Names
nSMase-2; nSMase2
UniProt Entry Name
NSMA2_HUMAN
Uniprot Description
SMPD3: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Belongs to the neutral sphingomyelinase family.
Protein type: EC 3.1.4.12; Phospholipase; Membrane protein, integral; Lipid Metabolism - sphingolipid
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: Golgi membrane; plasma membrane; Golgi cis cisterna
Molecular Function: sphingomyelin phosphodiesterase activity; metal ion binding
Biological Process: sphingolipid metabolic process; hemopoietic progenitor cell differentiation; glycosphingolipid metabolic process; sphingomyelin catabolic process; peptide hormone secretion; cell cycle
Research Articles on SMPD3
Similar Products
Product Notes
The SMPD3 smpd3 (Catalog #AAA9203057) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 137-165. The SMPD3 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SMPD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the SMPD3 smpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMPD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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