Western Blot (WB)
(Western Blot analysis of ADGRA2 in Human placenta tissue, Human bladder tissue, Human urinary bladder tissue lysates in order using ADGRA2 Rabbit pAb at dilution 1/1000)
Rabbit anti-Human, Mouse ADGRA2 Polyclonal Antibody | anti-ADGRA2 antibody
ADGRA2 Rabbit pAb
Gene Names
GPR124; TEM5
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
ADGRA2; Polyclonal Antibody; ADGRA2 Rabbit pAb; adhesion G protein-coupled receptor A2; TEM5; GPR124; anti-ADGRA2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
1.3mg/ml (varies by lot)
Applicable Applications for anti-ADGRA2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Immunogen
Synthetic peptide of human ADGRA2
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of ADGRA2 in Human placenta tissue, Human bladder tissue, Human urinary bladder tissue lysates in order using ADGRA2 Rabbit pAb at dilution 1/1000)
Western Blot (WB)
(Western Blot analysis of ADGRA2 in Human placenta tissue, Human bladder tissue, Human urinary bladder tissue lysates in order using ADGRA2 Rabbit pAb at dilution 1/1000)
Immunohistochemistry (IHC)
(Immunohistochemistry of ADGRA2 in paraffin-embedded Human liver cancer tissue using ADGRA2 Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm)
Immunohistochemistry (IHC)
(Immunohistochemistry of ADGRA2 in paraffin-embedded Human liver cancer tissue using ADGRA2 Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm)
Related Product Information for anti-ADGRA2 antibody
Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (PubMed:30026314). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
142,647 Da
NCBI Official Full Name
G-protein coupled receptor 124
NCBI Official Synonym Full Names
G protein-coupled receptor 124
NCBI Official Symbol
GPR124
NCBI Official Synonym Symbols
TEM5
NCBI Protein Information
G-protein coupled receptor 124; tumor endothelial marker 5
UniProt Protein Name
G-protein coupled receptor 124
Protein Family
UniProt Gene Name
GPR124
UniProt Synonym Gene Names
KIAA1531; TEM5
UniProt Entry Name
GP124_HUMAN
Uniprot Description
GPR124: Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; GPCR, family 2; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 8p11.23
Cellular Component: cell surface; plasma membrane; integral to membrane
Molecular Function: G-protein coupled receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; regulation of chemotaxis; central nervous system development; endothelial cell migration; sprouting angiogenesis; regulation of angiogenesis
Research Articles on ADGRA2
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Product Notes
The ADGRA2 gpr124 (Catalog #AAA4759607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADGRA2 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ADGRA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the ADGRA2 gpr124 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADGRA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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