Western Blot (WB)
(Western blot analysis of Adenylate Kinase 6 expression in Jurkat (A), HepG2 (B) whole cell lysates.)
Rabbit anti-Human Adenylate Kinase 6 Polyclonal Antibody | anti-AK6 antibody
Anti-Adenylate Kinase 6 Antibody
Western Blot (WB)
(Western blot analysis of Adenylate Kinase 6 expression in Jurkat (A), HepG2 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Adenylate Kinase 6 expression in Jurkat (A), HepG2 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Adenylate Kinase 6 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Adenylate Kinase 6 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Adenylate Kinase 6 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Adenylate Kinase 6 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the adenylate kinase family of enzymes. The protein has a nuclear localization and contains Walker A (P-loop) and Walker B motifs and a metal-coordinating residue. The protein may be involved in regulation of Cajal body formation. In human, AK6 and TAF9 (GeneID: 6880) are two distinct genes that share 5' exons. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Uniprot Description
TAFII31 iso2: a component of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is essential for mediating regulation of RNA polymerase transcription. TAFII31 binds to p53, the basal transcription factor GTF2B, and the acidic transactivator viral protein 16 (VP16). It is a coactivator for P53.
Protein type: Acetyltransferase; Transcription initiation complex; Kinase, other; EC 2.7.4.3; DNA-binding
Chromosomal Location of Human Ortholog: 5q13.2
Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex
Molecular Function: ATPase binding; DNA binding; histone acetyltransferase activity; p53 binding; protein binding; protein heterodimerization activity; transcription activator binding; transcription coactivator activity
Biological Process: box C/D snoRNP assembly; establishment and/or maintenance of chromatin architecture; gene expression; negative regulation of apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell growth; positive regulation of transcription from RNA polymerase II promoter; protein stabilization; response to DNA damage stimulus; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction
Research Articles on AK6
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Product Notes
The AK6 ak6 (Catalog #AAA8242350) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Adenylate Kinase 6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Adenylate Kinase 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the AK6 ak6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenylate Kinase 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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