Rabbit Adducin beta Polyclonal Antibody | anti-E497 antibody

Adducin beta (E497) Polyclonal Antibody

Cat. Num. AAA3002725

• Gene Names: ADD2; ADDB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Adducin beta; Polyclonal Antibody; Adducin beta (E497) Polyclonal Antibody; Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; beta-Adducin; Adducin1; Adducin-1; beta Adducin; Adducin-beta; anti-E497 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Adducin beta (E497) polyclonal antibody detects endogenous levels of Adducin beta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 726

• Applicable Applications for anti-E497 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human Adducin beta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Adducin beta (E497) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Adducin beta (E497) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Adducin beta (E497) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-E497 antibody

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta and gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin alpha and Adducin gamma is ubiquitous in contrast to the restricted expression of Adducin beta. Adducin beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.

NCBI and Uniprot Product Information

• NCBI GI #: 297307158
• NCBI GeneID: 119
• NCBI Accession #: NP_001171983.1
• NCBI GenBank Nucleotide #: NP_001171983.1
• UniProt Accession #: P35612
• Molecular Weight: ~ 81kDa
• NCBI Official Full Name: beta-adducin isoform a
• NCBI Official Synonym Full Names: adducin 2
• NCBI Official Symbol: ADD2
• NCBI Official Synonym Symbols: ADDB
• NCBI Protein Information: beta-adducin
• UniProt Protein Name: Beta-adducin
• UniProt Gene Name: ADD2
• UniProt Synonym Gene Names: ADDB
• UniProt Entry Name: ADDB_HUMAN

NCBI Description

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]

Uniprot Description

ADD2: a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Beta-adducin is restricted to brain and hematopoietic tissues. Three alternatively-spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: F-actin capping protein complex; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function: actin filament binding; calmodulin binding; protein homodimerization activity; spectrin binding; protein heterodimerization activity; actin binding; structural molecule activity

Biological Process: actin filament bundle formation; positive regulation of protein binding; hemopoiesis; protein complex assembly; barbed-end actin filament capping; actin cytoskeleton organization and biogenesis