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Western Blot (WB) (Western blot analysis of ADCY3 expression in Rat olfactory epithelium lysate; Rat frontal cortex lysate, The lane on the left is treated with the antigen-specific peptide.)

Rabbit ADCY3 Polyclonal Antibody | anti-ADCY3 antibody

ADCY3 Antibody

Gene Names
ADCY3; AC3; AC-III; BMIQ19
Reactivity
Human,Mouse,Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ADCY3; Polyclonal Antibody; ADCY3 Antibody; AC-III; AC3; ADCY3_HUMAN; adenylate cyclase 3; Adenylate cyclase; Adenylate cyclase type 3; Adenylate cyclase type III; Adenylyl cyclase 3; ATP pyrophosphate lyase; ATP pyrophosphate-lyase 3; olfactive type; anti-ADCY3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human,Mouse,Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ADCY3 antibody detects endogenous levels of total ADCY3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid;Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1145
Applicable Applications for anti-ADCY3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20000-1:40000

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human ADCY3,corresponding to a region within C-terminal amino acids.
Similarity
The protein contains two modules with six transmembrane helices each; both are required for catalytic activity. Isolated N-terminal or C-terminal modules have no catalytic activity,but when they are brought together, enzyme activity is restored. The active site is at the inter face of the two modules.Belongs to the adenylyl cyclase class-4/guanylylcyclase family.

Western Blot (WB)

(Western blot analysis of ADCY3 expression in Rat olfactory epithelium lysate; Rat frontal cortex lysate, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of ADCY3 expression in Rat olfactory epithelium lysate; Rat frontal cortex lysate, The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9603885 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9603885 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ADCY3 antibody
Mediates odorant detection (possibly) via modulation of intracellular cAMP concentration.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
109
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
129 kDa
NCBI Official Full Name
adenylate cyclase type 3 isoform 1
NCBI Official Synonym Full Names
adenylate cyclase 3
NCBI Official Symbol
ADCY3
NCBI Official Synonym Symbols
AC3; AC-III; BMIQ19
NCBI Protein Information
adenylate cyclase type 3
UniProt Protein Name
Adenylate cyclase type 3
Protein Family
UniProt Gene Name
ADCY3
UniProt Synonym Gene Names
KIAA0511; AC-III; AC3

NCBI Description

This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]

Uniprot Description

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Participates in signaling cascades triggered by odorant receptors via its function in cAMP biosynthesis. Required for the perception of odorants. Required for normal sperm motility and normal male fertility. Plays a role in regulating insulin levels and body fat accumulation in response to a high fat diet.

Research Articles on ADCY3

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Product Notes

The ADCY3 adcy3 (Catalog #AAA9603885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADCY3 Antibody reacts with Human,Mouse,Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADCY3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20000-1:40000 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the ADCY3 adcy3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADCY3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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