Immunohistochemistry (IHC)
(Anti-ADAMTS5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Rabbit ADAMTS5 Polyclonal Antibody | anti-ADAMTS5 antibody
Rabbit Polyclonal to Human ADAMTS5
Predicted Reactivity: Hamster
Predicted Reactivity: Hamster
Tissue Preperation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place precleaned and charged microscope sldies. Heat in a tissue-drying over for 45 Min. at 60°.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 min. each side at RT
Rehydration:
Wash slides 3 changes of 100% alcohol - 3 min. each side at RT
Wash slides 2 changes of 95% alcohol - 3 min. each side at RT
Wash slides 1 change of 80% alcohol - 3 min. each side at RT
Rinse slides in gentle running distilled water - 5 min. at RT
Antigen Retrieval:
Steam slides in 0.01 sodium citrate buffer, pH 6.0 at 99-100°C - 20 min
Remove from heat and let stand at RT in buffer - 20 min
Rinse in 1X TBS with tween (TBST) - 1 min. at RT
Immunostaining:
(do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 min. at RT
Drain protein block from slides, apply diluted primary antibody - 45 min. at RT
Rinse in 1X TBS with tween (TBST) - 1 min. at RT
Apply biotiylated secondary anitbody appropriate for the primary anitbody - 30 min. at RT
Rinse in 1X TBS with tween (TBST) - 1 min. at RT
Apply alkaline phosphatase streptavidin - 30 min. at RT
Rinse in 1X TBS with tween (TBST) - 1 min. at RT
Apply alkaline phosphatase chromogen substrate - 30 min. at RT
Wash slides in distilled water - 1 min. at RT.
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble)
Wash slides 2 changes of 80% alcohol - 1 min. each side at RT
Wash slides 2 changes of 95% alcohol - 1 min. each side at RT
Wash slides 3 changes of 100% alcohol - 1 min. each side at RT
Wash slides 3 changes of xylene - 1 min. each side at RT
Apply Covership.
Can be stored undiluted at 4°C for up to 1 month.
Avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Anti-ADAMTS5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-ADAMTS5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq, Jul 2008]
Uniprot Description
ADAMTS5: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Protein type: Protease; Extracellular matrix; EC 3.4.24.-; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 21q21.3
Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen; extracellular region
Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity
Biological Process: protein amino acid O-linked glycosylation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; defense response to bacterium; post-translational protein modification; proteolysis
Research Articles on ADAMTS5
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Product Notes
The ADAMTS5 adamts5 (Catalog #AAA247537) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human ADAMTS5 reacts with Bovine, Human, Monkey, Mouse, Pig, Rat Predicted Reactivity: Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's ADAMTS5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin). IHC-P (11 ug/ml). Researchers should empirically determine the suitability of the ADAMTS5 adamts5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAMTS5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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