Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human 293T Cells; Lane3: Human Hela Cells; Lane4: Porcine Pancreas Tissue.)
Rabbit anti-Human Serine Hydroxymethyltransferase 2 Polyclonal Antibody | anti-SHMT2 antibody
Polyclonal Antibody to Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human 293T Cells; Lane3: Human Hela Cells; Lane4: Porcine Pancreas Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human 293T Cells; Lane3: Human Hela Cells; Lane4: Porcine Pancreas Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SHMT2, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SHMT2, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Hela cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Hela cells))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: SHMT2 knockout 293T cell lysate; Predicted MW: 54kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human SHMT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: SHMT2 knockout 293T cell lysate; Predicted MW: 54kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human SHMT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Uniprot Description
SHMT2: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - glycine, serine and threonine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.1; Energy Metabolism - methane; Methyltransferase; Mitochondrial; Other Amino Acids Metabolism - cyanoamino acid
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: cytoplasm; extracellular exosome; microtubule cytoskeleton; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; nucleus
Molecular Function: amino acid binding; chromatin binding; glycine hydroxymethyltransferase activity; identical protein binding; L-allo-threonine aldolase activity; protein binding; pyridoxal phosphate binding
Biological Process: folic acid metabolic process; glycine biosynthetic process from serine; glycine metabolic process; L-serine biosynthetic process; L-serine metabolic process; one-carbon metabolic process; positive regulation of cell proliferation; protein homotetramerization; protein tetramerization; tetrahydrofolate metabolic process
Research Articles on SHMT2
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Product Notes
The SHMT2 shmt2 (Catalog #AAA2027992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Serine Hydroxymethyltransferase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the SHMT2 shmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serine Hydroxymethyltransferase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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