Western Blot (WB)
(Western Blot analysis of ADAMTS13 on SiHa cell (1), PC-3M cell (2) and A549 cell lysates using anti-ADAMTS13 antibody at 1/500 dilution.)
Rabbit anti-Human ADAMTS13 Polyclonal Antibody | anti-ADAMTS13 antibody
ADAMTS13 Polyclonal Antibody
Gene Names
ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
ADAMTS13; Polyclonal Antibody; ADAMTS13 Polyclonal Antibody; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease; C9orf8; A disintegrin and metalloproteinase with thrombospondin motifs 13; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13; A disintegrin like and metalloprotease with thrombospondin type 1 motif 13; ADAM metallopeptidase with thrombospondin type 1 motif 13; ADAM TS; ADAMTS 13; ADAMTS13 protein; ATS13; TTP; Von Willebrand factor cleaving protease; von Willebrand factor-cleaving protease; vWF cleaving protease; vWF CP; vWFCP; anti-ADAMTS13 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ADAMTS13 polyclonal antibody detects endogenous levels of ADAMTS13 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1371
Applicable Applications for anti-ADAMTS13 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein within human ADAMTS13 aa 150-350.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of ADAMTS13 on SiHa cell (1), PC-3M cell (2) and A549 cell lysates using anti-ADAMTS13 antibody at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of ADAMTS13 on SiHa cell (1), PC-3M cell (2) and A549 cell lysates using anti-ADAMTS13 antibody at 1/500 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ADAMTS13 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ADAMTS13 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-ADAMTS13 antibody
ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13)—also known as von Willebrand factor-cleaving protease (VWFCP)—is a zinc-containing metalloprotease enzyme that cleaves von Willebrand factor (vWf), a large protein involved in blood clotting. It is secreted in blood and degrades large vWf multimers, decreasing their activity. Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1, 3-glucose residue by the glucosyltransferase, B3GALTL.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Band Size: 154kDa
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 13
NCBI Official Symbol
ADAMTS13
NCBI Official Synonym Symbols
VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Gene Name
ADAMTS13
UniProt Synonym Gene Names
C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
NCBI Description
This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.
Research Articles on ADAMTS13
Similar Products
Product Notes
The ADAMTS13 adamts13 (Catalog #AAA3012200) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAMTS13 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADAMTS13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ADAMTS13 adamts13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAMTS13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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