Rabbit anti-Human ADAMTS13 Polyclonal Antibody | anti-ADAMTS13 antibody

ADAMTS13, ID (von Willebrand Factor-cleaving Protease, vWF-cleaving Protease, vWF-CP, A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13, ADAMTS-13, ADAM-TS 13, ADAM-TS13, UNQ6102/PRO20085, C9orf8)

Cat. Num. AAA630150

• Gene Names: ADAMTS13; TTP; VWFCP; C9orf8; vWF-CP; FLJ42993; MGC118899; MGC118900; DKFZp434C2322
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ADAMTS13; Polyclonal Antibody; ID (von Willebrand Factor-cleaving Protease; vWF-cleaving Protease; vWF-CP; A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13; ADAMTS-13; ADAM-TS 13; ADAM-TS13; UNQ6102/PRO20085; C9orf8); Anti -ADAMTS13; anti-ADAMTS13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADAMTS13.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ADAMTS13 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the center region of human ADAMTS13 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS630150. ADAMTS13 (arrow) was detected using MBS630150.)

Immunohistochemistry (IHC)

(Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma using MBS630150, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Related Product Information for anti-ADAMTS13 antibody

ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.

Product Categories/Family for anti-ADAMTS13 antibody

Antibodies; Abs to Matrix Metalloproteinases

References

ADAMTS13. Christian R. Robinson, Ina Laura Pieper, Venkateswarlu Kanamarlapudi (2017)

NCBI and Uniprot Product Information

• NCBI GI #: 73695936
• NCBI GeneID: 11093
• NCBI Accession #: NP_620596.2
• NCBI GenBank Nucleotide #: NM_139027.3
• UniProt Accession #: Q6QNA4; Q6QNA5; Q6QNA6; Q6QNA7; Q76LX8; Q9UGQ1
• Molecular Weight: 6,844 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 13
• NCBI Official Symbol: ADAMTS13
• NCBI Official Synonym Symbols: TTP; VWFCP; C9orf8; vWF-CP; FLJ42993; MGC118899; MGC118900; DKFZp434C2322
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS13; ADAMTS-13; ADAM-TS 13; OTTHUMP00000022496; OTTHUMP00000022497; OTTHUMP00000022498; vWF-cleaving protease; von Willebrand factor-cleaving protease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13
• UniProt Protein Name: Von Willebrand factor-cleaving protease
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS13
• UniProt Entry Name: Q6QNA4_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.87; Calcium-binding; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen

Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: integrin-mediated signaling pathway; platelet activation; protein amino acid O-linked glycosylation; cellular protein metabolic process; glycoprotein metabolic process; response to toxin; cell-matrix adhesion; peptide catabolic process; protein processing; post-translational protein modification; proteolysis

Disease: Thrombotic Thrombocytopenic Purpura, Congenital