Rabbit anti-Human TRIM13 Polyclonal Antibody | anti-TRIM13 antibody

TRIM13, NT (TRIM13, LEU5, RFP2, RNF77, E3 ubiquitin-protein ligase TRIM13, B Cell chronic lymphocytic leukemia tumor suppressor Leu5, Leukemia-associated protein 5, Putative tumor suppressor RFP2, RING finger protein 77, Ret finger protein 2, Tripartite m

Cat. Num. AAA6358147

• Gene Names: TRIM13; CAR; LEU5; RFP2; DLEU5; RNF77
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM13; Polyclonal Antibody; NT (TRIM13; LEU5; RFP2; RNF77; E3 ubiquitin-protein ligase TRIM13; B Cell chronic lymphocytic leukemia tumor suppressor Leu5; Leukemia-associated protein 5; Putative tumor suppressor RFP2; RING finger protein 77; Ret finger protein 2; Tripartite m; B-cell chronic lymphocytic leukemia tumor suppressor Leu5; Tripartite motif-containing protein 13; anti-TRIM13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 407

• Applicable Applications for anti-TRIM13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TRIM13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human TRIM13.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRIM13 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TRIM13 antibody detected the TRIM13 protein (arrow).)

Related Product Information for anti-TRIM13 antibody

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia. Multiple alternatively spliced transcript variants have been found for this gene.

Product Categories/Family for anti-TRIM13 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

Lerner, M., et al. Mol. Biol. Cell 18(5):1670-1682(2007). Skoblov, M., et al. Biochem. Biophys. Res. Commun. 342(3):859-866(2006). Corcoran, M.M., et al. Genes Chromosomes Cancer 40(4):285-297(2004). Dunham, A., et al. Nature 428(6982):522-528(2004). van Everdink, W.J., et al. Cancer Genet. Cytogenet. 146(1):48-57(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 47132521
• NCBI GeneID: 10206
• NCBI Accession #: NP_005789.2; NP_434698.1; NP_001007279.1; NP_998755.1
• NCBI GenBank Nucleotide #: NP_005789.2
• UniProt Accession #: O60858
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM13 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 13
• NCBI Official Symbol: TRIM13
• NCBI Official Synonym Symbols: CAR; LEU5; RFP2; DLEU5; RNF77
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM13
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM13
• Protein Family: Tripartite motif containing
• UniProt Gene Name: TRIM13
• UniProt Synonym Gene Names: LEU5; RFP2; RNF77
• UniProt Entry Name: TRI13_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM13: E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Membrane protein, integral; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Tumor suppressor

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: endoplasmic reticulum membrane; cytoplasm; integral to membrane

Molecular Function: protein binding; signal transducer activity; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; negative regulation of viral transcription; ER-associated protein catabolic process; anatomical structure morphogenesis; protein autoubiquitination; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to gamma radiation; innate immune response; signal transduction; positive regulation of macroautophagy; activation of NF-kappaB transcription factor