Mouse ADAM DEC1, ADAMDEC1 ELISA Kit | ADAMDEC1 elisa kit
Mouse ADAM DEC1, ADAMDEC1 ELISA Kit
Reactivity
Mouse
Synonyms
ADAM DEC1; ADAMDEC1; Mouse ADAM DEC1; ADAMDEC1 ELISA Kit; ADAMDEC1 elisa kit
Reactivity
Mouse
Sequence Length
391
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
20-4500ng/L
Sensitivity
9.29ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for ADAMDEC1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse ADAM DEC1 (also known as ADAMDEC1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ADAMDEC1 antibody. ADAMDEC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ADAMDEC1 Antibody is added and binds to ADAMDEC1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAMDEC1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ADAMDEC1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ADAMDEC1 antibody. ADAMDEC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ADAMDEC1 Antibody is added and binds to ADAMDEC1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAMDEC1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ADAMDEC1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ADAM DEC1 isoform 2
UniProt Protein Name
ADAM DEC1
Protein Family
UniProt Gene Name
ADAMDEC1
UniProt Synonym Gene Names
ADAM-like protein decysin-1
UniProt Entry Name
ADEC1_HUMAN
Uniprot Description
Function: May play an important role in the control of the immune response and during pregnancy
By similarity.
Cofactor: Binds 1 zinc ion per subunit
By similarity.
Subcellular location: Secreted
By similarity.
Tissue specificity: Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in monocyte and highly expressed in macrophage. Absent in immature DC. Ref.1 Ref.5
Induction: Induced during DC maturation and up-regulated in response to T-cell signals. In macrophage up-regulated by bacterial lipopolysaccharides (LPS). Up-regulated by 1-alpha,25-dihydroxyvitamin D3 during differentiation of primary monocyte into macrophage. Ref.1 Ref.5
Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.
