Rabbit ADAM10 Polyclonal Antibody | anti-ADAM10 antibody

ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10) (FITC)

Cat. Num. AAA6461458

• Gene Names: ADAM10; kuz; AD10; MADM; CD156c; HsT18717
• Reactivity: Human, Bovine, Mouse
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADAM10; Polyclonal Antibody; ADAM 10 (Kuzbanian; KUZ; MADM; a Disintegrin and Metalloprotease 10) (FITC); anti-ADAM10 antibody

• Host: Rabbit
• Reactivity: Human, Bovine, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADAM 10, C-terminus. Species Crossreactivity: bovine and mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADAM10 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: A previous lot of this antibody was used to detect ADAM10 in formalin-fixed, paraffin-embedded renal tissue and renal cell carcinoma tissue (Gutwein, P., et al., 2009).; Applications are based on unconjugated antibody.; WB: 1:500-1:2000 detected ADAM 10 on 10ug of RAW264.7 lysates. RAW264.7 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and chemiluminesence detection system.
• Immunogen: Raised against a peptide corresponding to aa732-748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid. Species sequence homology: rat
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Testing Data

(AW264.7 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with A0859-20 (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and chemiluminesence detection system. Arrow indicates protein ADAM 10 (~85kD).)

Product Categories/Family for anti-ADAM10 antibody

Antibodies; Matrix Metalloproteinases

References

1. Gutwein, P., et al. EUROPEAN JOURNAL OF CANCER. 45(2009):478-489. 2. Farris W., et al., (2003). PNAS. 100 (7):4162-4167. 3. Slack B.E., et al. (2001). Biochem. J. 357:787-794. 4. Qi, H., et al. (1999). Science. 283:91-4. 5. Pan, D. (1997). Cell. 90: 271-80. 6. Rosendahl, M.S. (1997). J. Biol. Chem. 272:24588-93. 7. Howard, L., et al. (1996). Biochem J. 317:45.

NCBI and Uniprot Product Information

• NCBI GI #: 2393947
• NCBI GeneID: 102
• NCBI Accession #: AAC51766.1
• NCBI GenBank Nucleotide #: NM_001110.2
• UniProt Accession #: O14672; Q10742; Q92650
• Molecular Weight: 84,142 Da
• NCBI Official Full Name: ADAM10
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: ADAM10
• NCBI Official Synonym Symbols: kuz; AD10; MADM; CD156c; HsT18717
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10; CDw156; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM10
• UniProt Synonym Gene Names: KUZ; MADM; ADAM 10
• UniProt Entry Name: ADA10_HUMAN

NCBI Description

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Cell adhesion; Vesicle; Motility/polarity/chemotaxis; Protease; Membrane protein, integral; EC 3.4.24.81; Cell surface

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; cell surface; focal adhesion; membrane; intracellular membrane-bound organelle; cytoplasm; postsynaptic density; plasma membrane; integral to membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; protein kinase binding; SH3 domain binding; receptor binding

Biological Process: integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; protein amino acid phosphorylation; positive regulation of cell growth; extracellular matrix disassembly; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; cell-cell signaling; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18