Western Blot (WB)
(Western blot analysis of ADAM33 in Human Prostate lysate (35μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide using ADAM33 antibody (0.3μg/ml).)
Goat anti-Human ADAM 33 Polyclonal Antibody | anti-ADAM33 antibody
ADAM 33 (A Disintegrin and Metalloprotease 33, A Disintegrin and Metalloproteinase Domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, Disintegrin and Reprolysin Metalloproteinase Family Protein, Metalloprotease Disintegrin)
Gene Names
ADAM33; FLJ35308; FLJ36751; MGC71889; C20orf153; DJ964F7.1; MGC149823; DKFZp434K0521; ADAM33
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
ADAM 33; Polyclonal Antibody; ADAM 33 (A Disintegrin and Metalloprotease 33; A Disintegrin and Metalloproteinase Domain 33; HGNC:15478; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; MGC71889; Disintegrin and Reprolysin Metalloproteinase Family Protein; Metalloprotease Disintegrin); Anti -ADAM 33 (A Disintegrin and Metalloprotease 33; anti-ADAM33 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human ADAM33 at ~80-90kD and strong ladder from 26 to 38kD in Human Prostate lysates after 0.3ug/ml antibody staining. This protein has a calculated MW of 87.7kD according to NP_079496.1. This antibody is expected to recognize isoform alpha (NP_079496) and not isoform beta (NP_694882).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-ADAM33 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:64000
Western Blot: 0.1-0.3ug/ml
Dilution: ELISA: 1:64000
Western Blot: 0.1-0.3ug/ml
Immunogen
Synthetic peptide corresponding to human ADAM33.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of ADAM33 in Human Prostate lysate (35μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide using ADAM33 antibody (0.3μg/ml).)
Western Blot (WB)
(Western blot analysis of ADAM33 in Human Prostate lysate (35μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide using ADAM33 antibody (0.3μg/ml).)
Related Product Information for anti-ADAM33 antibody
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Product Categories/Family for anti-ADAM33 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
87,668 Da
NCBI Official Full Name
ADAM metallopeptidase domain 33
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 33
NCBI Official Symbol
ADAM33
NCBI Official Synonym Symbols
FLJ35308; FLJ36751; MGC71889; C20orf153; DJ964F7.1; MGC149823; DKFZp434K0521; ADAM33
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 33; ADAM 33; a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; disintegrin and reprolysin metalloproteinase family protein
UniProt Protein Name
ADAM metallopeptidase domain 33
Protein Family
UniProt Gene Name
ADAM33
UniProt Entry Name
A2A2L3_HUMAN
NCBI Description
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
Uniprot Description
Sequence similarities: Contains 1 disintegrin domain. RuleBase RU004074 SAAS SAAS001762Contains 1 peptidase M12B domain. RuleBase RU004141 SAAS SAAS001762
Research Articles on ADAM33
1. The polymorphism of T(1) locus allele in ADAM33 gene is associated with the susceptibility to asthma in South China Han population.
2. Our findings suggest a relevant role of ADAM33 in thepathogenesis of asthma in this population in Cartagena, Colombia.
3. preliminary results suggest an association between ADAM33 polymorphisms and COPD in a Chinese Han population
4. identification of ADAM33 as a psoriasis susceptibility gene identified by positional cloning in an outbred population should provide insights into the pathogenesis and natural history of this common disease
5. These results support the note that Th2 cytokines can up-regulate the expression of ADAM33 mRNA and ADAM33 may play an important role in the development of airway remodeling in allergen-induced chronic airway inflammation.
6. Adam33/Adam33 shows 2 significant increments in expression during lung morphogenesis, suggesting important developmental regulation.
7. The SNPs (V4 G/C, T2 A/G, T1 G/A, and Q - 1A/G) of the ADAM33 gene may be the causal variants in allergic rhinitis and asthma
8. Smoke exposure interacts with ADAM33 polymorphisms in the development of lung function and hyperresponsiveness.
9. First epidemiological study on the relationship between polymorphisms in the ADAM33 gene and the risk of atopic dermatitis. The rs2853209 SNP in the ADAM33 gene was significantly associated with the risk of AD in Japanese children.
10. Adam33 polymorphisms are associated with chronic obstructive pulmonary disease and lung function in long-term tobacco smokers.
2. Our findings suggest a relevant role of ADAM33 in thepathogenesis of asthma in this population in Cartagena, Colombia.
3. preliminary results suggest an association between ADAM33 polymorphisms and COPD in a Chinese Han population
4. identification of ADAM33 as a psoriasis susceptibility gene identified by positional cloning in an outbred population should provide insights into the pathogenesis and natural history of this common disease
5. These results support the note that Th2 cytokines can up-regulate the expression of ADAM33 mRNA and ADAM33 may play an important role in the development of airway remodeling in allergen-induced chronic airway inflammation.
6. Adam33/Adam33 shows 2 significant increments in expression during lung morphogenesis, suggesting important developmental regulation.
7. The SNPs (V4 G/C, T2 A/G, T1 G/A, and Q - 1A/G) of the ADAM33 gene may be the causal variants in allergic rhinitis and asthma
8. Smoke exposure interacts with ADAM33 polymorphisms in the development of lung function and hyperresponsiveness.
9. First epidemiological study on the relationship between polymorphisms in the ADAM33 gene and the risk of atopic dermatitis. The rs2853209 SNP in the ADAM33 gene was significantly associated with the risk of AD in Japanese children.
10. Adam33 polymorphisms are associated with chronic obstructive pulmonary disease and lung function in long-term tobacco smokers.
Similar Products
Product Notes
The ADAM33 adam33 (Catalog #AAA620473) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The ADAM 33 (A Disintegrin and Metalloprotease 33, A Disintegrin and Metalloproteinase Domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, Disintegrin and Reprolysin Metalloproteinase Family Protein, Metalloprotease Disintegrin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADAM 33 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:64000 Western Blot: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the ADAM33 adam33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAM 33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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