Rabbit anti-Human ACY2 Polyclonal Antibody | anti-ACY2 antibody

ACY2, NT (Aminoacylase-2, ACY-2, Aspartoacylase, ASP, ASPA) (APC)

Cat. Num. AAA6462635

• Gene Names: ASPA; ASP; ACY2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ACY2; Polyclonal Antibody; NT (Aminoacylase-2; ACY-2; Aspartoacylase; ASP; ASPA) (APC); anti-ACY2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ASPA.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ACY2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ASPA (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ASPA Antibody (N-term) in MCF7 cell line lysates (35ug/lane). ASPA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of ASPA (arrow) using rabbit polyclonal ASPA Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ASPA Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ACY2 antibody

Antibodies; Enzymes

References

1. Bitto, E., et.al., Proc. Natl. Acad. Sci. USA 104(2), 456-461 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4557335
• NCBI GeneID: 443
• NCBI Accession #: NP_000040.1
• NCBI GenBank Nucleotide #: NM_000049.2
• UniProt Accession #: P45381
• NCBI Official Full Name: aspartoacylase
• NCBI Official Synonym Full Names: aspartoacylase
• NCBI Official Symbol: ASPA
• NCBI Official Synonym Symbols: ASP; ACY2
• NCBI Protein Information: aspartoacylase; ACY-2; aminoacylase 2; aminoacylase-2
• UniProt Protein Name: Aspartoacylase
• UniProt Gene Name: ASPA
• UniProt Synonym Gene Names: ACY2; ASP; ACY-2
• UniProt Entry Name: ACY2_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ASPA: Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids. Defects in ASPA are the cause of Canavan disease (CAND); also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average. Belongs to the AspA/AstE family. Aspartoacylase subfamily.

Protein type: Hydrolase; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - histidine; EC 3.5.1.15

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; hydrolase activity, acting on ester bonds; aspartoacylase activity; aminoacylase activity

Biological Process: myelination in the central nervous system; aspartate catabolic process; positive regulation of oligodendrocyte differentiation

Disease: Canavan Disease