Human Aspartoacylase ELISA Kit | ASPA elisa kit

Human Aspartoacylase ELISA Kit

Cat. Num. AAA288790

• Gene Names: ASPA; ASP; ACY2
• Reactivity: Human
• Synonyms: Aspartoacylase; Human Aspartoacylase ELISA Kit; Aminoacylase-2; ACY-2; ASPA; ACY2; ASP; 3.5.1.15; ASPA elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Tyrosine-protein kinase CSK
• Sequence Length: 313

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: <0.43 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.5%
• Inter-assay Precision: Inter-Assay CV: <=10.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASPA elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557335
• NCBI GeneID: 443
• NCBI Accession #: NP_000040.1
• NCBI GenBank Nucleotide #: NM_000049.2
• UniProt Accession #: P45381
• Molecular Weight: 35,735 Da
• NCBI Official Full Name: aspartoacylase
• NCBI Official Synonym Full Names: aspartoacylase
• NCBI Official Symbol: ASPA
• NCBI Official Synonym Symbols: ASP; ACY2
• NCBI Protein Information: aspartoacylase
• UniProt Protein Name: Aspartoacylase
• Protein Family: Aspartoacylase
• UniProt Gene Name: ASPA
• UniProt Synonym Gene Names: ACY2; ASP; ACY-2
• UniProt Entry Name: ACY2_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ASPA: Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids. Defects in ASPA are the cause of Canavan disease (CAND); also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average. Belongs to the AspA/AstE family. Aspartoacylase subfamily.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Hydrolase; Amino Acid Metabolism - histidine; EC 3.5.1.15

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: aminoacylase activity; aspartoacylase activity; hydrolase activity, acting on ester bonds; metal ion binding; protein binding

Biological Process: amino acid biosynthetic process; aspartate catabolic process

Disease: Canavan Disease