Rabbit anti-Human, Mouse ACVR1B Polyclonal Antibody | anti-ACVR1B antibody

ACVR1B, ID (Activin Receptor Type-1B, Activin Receptor Type IB, Serine/Threonine-protein Kinase Receptor R2, SKR2, Activin Receptor-like Kinase 4, ACVRLK4, ALK4) (HRP)

Cat. Num. AAA6461327

• Gene Names: ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ACVR1B; Polyclonal Antibody; ID (Activin Receptor Type-1B; Activin Receptor Type IB; Serine/Threonine-protein Kinase Receptor R2; SKR2; Activin Receptor-like Kinase 4; ACVRLK4; ALK4) (HRP); anti-ACVR1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACVR1B. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ACVR1B antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: 1:1,000; WB: 1:100-1:500; FC/FACS: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ACVR1B (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(The anti-ACVR1B Pab is used in Western blot to detect ACVR1B in mouse kidney tissue lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using Activin A Receptor Type IB (ACVR1B) Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(ACVR1B Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ACVR1B antibody detected the ACVR1B protein (arrow).)

Product Categories/Family for anti-ACVR1B antibody

Antibodies; Activin Receptors

References

1. Harrison, C.A., et al., J. Biol. Chem. 278(23):21129-21135 (2003). 2. Mukasa, C., et al., Endocrinology 144(4):1603-1611 (2003). 3. Danila, D.C., et al., J. Clin. Endocrinol. Metab. 87(10):4741-4746 (2002). 4. Schneider-Kolsky, M.E., et al., Placenta 23(4):294-302 (2002). 5. Roijer, E., et al., Mamm. Genome 9(3):266-268 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4757720
• NCBI GeneID: 91
• NCBI Accession #: NP_004293.1
• NCBI GenBank Nucleotide #: NM_004302.4
• UniProt Accession #: P36896; Q15479; Q15480; Q15481; Q15482; B7Z5L8; B7Z5W5
• Molecular Weight: 56,807 Da
• NCBI Official Full Name: activin receptor type-1B isoform a
• NCBI Official Synonym Full Names: activin A receptor, type IB
• NCBI Official Symbol: ACVR1B
• NCBI Official Synonym Symbols: ALK4; SKR2; ACTRIB; ACVRLK4
• NCBI Protein Information: activin receptor type-1B; activin receptor-like kinase 4; activin A receptor, type II-like kinase 4; serine/threonine-protein kinase receptor R2
• UniProt Protein Name: Activin receptor type-1B
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR1B
• UniProt Synonym Gene Names: ACVRLK4; ALK4; ACTR-IB; ALK-4; SKR2
• UniProt Entry Name: ACV1B_HUMAN

NCBI Description

This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

ALK4: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.11.30; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; activin receptor complex; receptor complex

Molecular Function: activin receptor activity; metal ion binding; transmembrane receptor protein serine/threonine kinase activity; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; ubiquitin protein ligase binding; growth factor binding; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: development of primary female sexual characteristics; central nervous system development; in utero embryonic development; positive regulation of erythrocyte differentiation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; activin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of activin receptor signaling pathway; hair follicle development; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; G1/S transition of mitotic cell cycle