Rabbit anti-Human, Mouse Actin, beta Polyclonal Antibody | anti-ACTB antibody

Actin, beta

Cat. Num. AAA626179

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Actin; beta; Polyclonal Antibody; Anti -Actin; anti-ACTB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Detects a single clean band at 40kD representing actin. Species Crossreactivity: Crossreacts with Human and mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Tris-citrate/phosphate pH 7 to 8

• Applicable Applications for anti-ACTB antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 40kD band is detected in HeLa whole cell lysate and mouse NIH3T3 cells.
• Immunogen: The epitope that this antibody recognizes maps to the N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709. The N-terminus of beta actin is highly conserved with gamma actin. Initial tests show that this antibody recognizes both beta and gamma actin.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ACTB antibody

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.

Product Categories/Family for anti-ACTB antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 16359158
• NCBI GeneID: 60
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: 41,737 Da
• NCBI Official Full Name: Actin, beta
• NCBI Official Synonym Full Names: actin, beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Actin
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; postsynaptic density; ribonucleoprotein complex; cytosol; nucleoplasm; cytoskeleton; membrane; axon; nuclear chromatin; cytoplasm; plasma membrane

Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; retinal homeostasis; 'de novo' posttranslational protein folding; substantia nigra development; cellular protein metabolic process; ephrin receptor signaling pathway; ATP-dependent chromatin remodeling; innate immune response; cell motility; blood coagulation; vascular endothelial growth factor receptor signaling pathway

Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1