Western Blot (WB)
(Fig. Western blot analysis of Nicotiana tabacum leaf (1), Nicotiana tabacum root (2), Nicotiana seed (3), Oryza sativa flower (4), Oryza sativa seed (5), Oryza sativa leaf (6), Hordeum vulgare seed (7), Hordeum vulgare leaves (8), diluted at 1:3000. Secondary antibody was diluted at 1:20000.)
Rabbit Actin Polyclonal Antibody | anti-ACTB antibody
Plant-Actin Polyclonal Antibody
Reactivity
Arabidopsis, Nicotiana Tabacum, Oryza Sativa, Hordeum Vulgare
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Synonyms
Actin; Polyclonal Antibody; Plant-Actin Polyclonal Antibody; Rabbit Anti-Plant-actin Polyclonal Antibody; cytoplasmic 1; Beta-actin; anti-ACTB antibody
Host
Rabbit
Reactivity
Arabidopsis, Nicotiana Tabacum, Oryza Sativa, Hordeum Vulgare
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous plant-actin protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form/Format
Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-ACTB antibody
Western Blot (WB)
Application Notes
WB: 1:3000
Immunogen
Recombinant Protein
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig. Western blot analysis of Nicotiana tabacum leaf (1), Nicotiana tabacum root (2), Nicotiana seed (3), Oryza sativa flower (4), Oryza sativa seed (5), Oryza sativa leaf (6), Hordeum vulgare seed (7), Hordeum vulgare leaves (8), diluted at 1:3000. Secondary antibody was diluted at 1:20000.)
Western Blot (WB)
(Fig. Western blot analysis of Nicotiana tabacum leaf (1), Nicotiana tabacum root (2), Nicotiana seed (3), Oryza sativa flower (4), Oryza sativa seed (5), Oryza sativa leaf (6), Hordeum vulgare seed (7), Hordeum vulgare leaves (8), diluted at 1:3000. Secondary antibody was diluted at 1:20000.)
Related Product Information for anti-ACTB antibody
Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Similar Products
Product Notes
The ACTB (Catalog #AAA9700917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Plant-Actin Polyclonal Antibody reacts with Arabidopsis, Nicotiana Tabacum, Oryza Sativa, Hordeum Vulgare and may cross-react with other species as described in the data sheet. AAA Biotech's Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:3000. Researchers should empirically determine the suitability of the ACTB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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