Rabbit anti-Human ACTG1 Polyclonal Antibody | anti-ACTG1 antibody

ACTG1, ID (Actin, Cytoplasmic 2, Gamma-actin, Actin, Cytoplasmic 2, N-terminally Processed, ACTG, ACTB) (HRP)

Cat. Num. AAA6461206

• Gene Names: ACTG1; ACT; ACTG; BRWS2; DFNA20; DFNA26; HEL-176
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ACTG1; Polyclonal Antibody; ID (Actin; Cytoplasmic 2; Gamma-actin; Actin; N-terminally Processed; ACTG; ACTB) (HRP); anti-ACTG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACTG1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ACTG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ACTG1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of ACTG1 Antibody (Center) in K562, HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ACTG1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ACTG1 antibody

Antibodies; Actin Related Proteins

References

1. de Heer, A.M., Ann. Otol. Rhinol. Laryngol. 118(5), 382-390 (2009). 2. Mouilleron, S., EMBO J. 27(23), 3198-3208 (2008). 3. Liu, P., J Genet Genomics 35(9), 553-558 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4501887
• NCBI GeneID: 71
• NCBI Accession #: NP_001605
• NCBI GenBank Nucleotide #: NM_001614.3
• UniProt Accession #: P63261; P02571; P14104; P99022; Q5U032; Q96E67; A8K7C2
• Molecular Weight: 41,793 Da
• NCBI Official Full Name: actin, cytoplasmic 2
• NCBI Official Synonym Full Names: actin gamma 1
• NCBI Official Symbol: ACTG1
• NCBI Official Synonym Symbols: ACT; ACTG; BRWS2; DFNA20; DFNA26; HEL-176
• NCBI Protein Information: actin, cytoplasmic 2
• UniProt Protein Name: Actin, cytoplasmic 2
• Protein Family: Actin
• UniProt Gene Name: ACTG1
• UniProt Synonym Gene Names: ACTG
• UniProt Entry Name: ACTG_HUMAN

NCBI Description

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

Uniprot Description

ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: filamentous actin; extracellular space; cytoskeleton; myofibril; membrane; plasma membrane; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding

Biological Process: axon guidance; retinal homeostasis; intercellular junction assembly and maintenance; ephrin receptor signaling pathway; innate immune response; sarcomere organization; cell motility; vascular endothelial growth factor receptor signaling pathway

Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20