Human Ornithine aminotransferase, mitochondrial ELISA Kit | OAT elisa kit

Human Ornithine aminotransferase, mitochondrial ELISA Kit

Cat. Num. AAA762133

• Gene Names: OAT; OKT; GACR; HOGA; OATASE
• Reactivity: Human
• Synonyms: Ornithine aminotransferase; mitochondrial; Human Ornithine aminotransferase; mitochondrial ELISA Kit; OAT/Ornithine aminotransferase; mitochondrial/Ornithine delta-aminotransferase/Ornithine--oxo-acid aminotransferase; OAT elisa kit

• Reactivity: Human
• Sequence Length: 301

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for OAT elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human OAT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OAT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OAT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human OAT can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 284507298
• NCBI GeneID: 4942
• NCBI Accession #: NP_001165285.1
• NCBI GenBank Nucleotide #: NM_001171814.1
• Molecular Weight: 32,853 Da
• NCBI Official Full Name: ornithine aminotransferase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: ornithine aminotransferase
• NCBI Official Symbol: OAT
• NCBI Official Synonym Symbols: OKT; GACR; HOGA; OATASE
• NCBI Protein Information: ornithine aminotransferase, mitochondrial
• UniProt Protein Name: Ornithine aminotransferase, mitochondrial
• UniProt Gene Name: OAT
• UniProt Entry Name: OAT_HUMAN

NCBI Description

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]

Uniprot Description

OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.6.1.13; Transferase; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: identical protein binding; ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; arginine catabolic process to glutamate; arginine catabolic process to proline via ornithine; visual perception

Disease: Gyrate Atrophy Of Choroid And Retina