Rabbit anti-Human Aconitase 1 Polyclonal Antibody | anti-ACO1 antibody

Aconitase 1 (ACO1, ACONS, IREB1, IREBP, IREBP1, IRP1, Aconitate Hydratase, Citrate Hydro-lyase, Ferritin Repressor Protein, Iron Regulatory Protein 1, Iron-responsive Element Binding Protein 1) (Biotin)

Cat. Num. AAA6461094

• Gene Names: ACO1; IRP1; ACONS; IREB1; IREBP; IREBP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified
• Synonyms: Aconitase 1; Polyclonal Antibody; Aconitase 1 (ACO1; ACONS; IREB1; IREBP; IREBP1; IRP1; Aconitate Hydratase; Citrate Hydro-lyase; Ferritin Repressor Protein; Iron Regulatory Protein 1; Iron-responsive Element Binding Protein 1) (Biotin); anti-ACO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACO1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ACO1 antibody: Western Blot (WB)
• Application Notes: For maximum recovery of product, 11c7entrifuge the original vial after thawing and prior to removing the cap.; Applications are based on unconjugated antibody.
• Immunogen: ACO1 (NP_002188.1, 1-889aa) full-length human protein.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ACO1 antibody

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Product Categories/Family for anti-ACO1 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 8659555
• NCBI GeneID: 48
• NCBI Accession #: NP_002188.1
• NCBI GenBank Nucleotide #: NM_002197.2
• UniProt Accession #: P21399; Q14652; Q5VZA7; D3DRK7
• Molecular Weight: 98,399 Da
• NCBI Official Full Name: cytoplasmic aconitate hydratase
• NCBI Official Synonym Full Names: aconitase 1, soluble
• NCBI Official Symbol: ACO1
• NCBI Official Synonym Symbols: IRP1; ACONS; IREB1; IREBP; IREBP1
• NCBI Protein Information: cytoplasmic aconitate hydratase; IRE-BP 1; citrate hydro-lyase; iron regulatory protein 1; ferritin repressor protein; aconitate hydratase, cytoplasmic; iron-responsive element binding protein 1; iron-responsive element-binding protein 1
• UniProt Protein Name: Cytoplasmic aconitate hydratase
• Protein Family: Aconitate hydratase
• UniProt Gene Name: ACO1
• UniProt Synonym Gene Names: IREB1; Aconitase; IRP1; IRE-BP 1
• UniProt Entry Name: ACOC_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: Translation; RNA-binding; Lyase; Endoplasmic reticulum; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: Golgi apparatus; mitochondrion; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; RNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; aconitate hydratase activity

Biological Process: regulation of translation; cellular iron ion homeostasis; tricarboxylic acid cycle; citrate metabolic process; response to iron(II) ion; intestinal absorption; post-embryonic development