Rabbit anti-Human Ack1 Polyclonal Antibody | anti-TNK2 antibody

Ack1, phosphorylated (Tyr284) (TNK2, Tyrosine Kinase, non-receptor 2, Tyrosine Kinase non-receptor protein 2, ACK, ACK1, Activated CDC42 kinase 1, FLJ44758, FLJ45547, p21cdc42Hs)

Cat. Num. AAA611868

• Gene Names: TNK2; ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Ack1; Polyclonal Antibody; phosphorylated (Tyr284) (TNK2; Tyrosine Kinase; non-receptor 2; Tyrosine Kinase non-receptor protein 2; ACK; ACK1; Activated CDC42 kinase 1; FLJ44758; FLJ45547; p21cdc42Hs); Anti -Ack1; anti-TNK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACK1 phosphorylated at Tyrosine residue 284 at ~114kD.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, 30% glycerol.

• Applicable Applications for anti-TNK2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:10,000. Detects ACK1 phosphorylated on Tyrosine residue 284 in COS7 cells transfected with wild type ACK1 but not in COS7 cells transfected with ACK1(Y284F). Lysates from COS7 cells, transfected with either wild type ACK1 or mutant ACK1 (Y284F), were resolved by electrophoresis, transferred to PVDF (Immobilon-P), and probed with A0650-01 for 2 hours at RT. Proteins were visualized via HRP and chemiluminescent detection.
• Immunogen: Synthetic peptide corresponding to amino acids surrounding Tyr284 of human ACK1 conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot Analysis: Lysates from COS7 cells transfected with either wild type ACK1 (Lane 1) or mutant ACK1 (Y284F) (Lane 2) was resolved by electrophoresis, transferred to PVDF (Immobilon-P), and probed with MBS611868 for 2 hours at RT. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-ACK1.)

Related Product Information for anti-TNK2 antibody

Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region. Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility. Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer.

Product Categories/Family for anti-TNK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 58331191
• NCBI GeneID: 10188
• NCBI Accession #: NP_001010938.1
• NCBI GenBank Nucleotide #: NM_001010938.1
• UniProt Accession #: Q07912; Q6ZMQ0; Q8N6U7; Q96H59; Q59FX1
• Molecular Weight: 114,569 Da
• NCBI Official Full Name: activated CDC42 kinase 1 isoform 2
• NCBI Official Synonym Full Names: tyrosine kinase, non-receptor, 2
• NCBI Official Symbol: TNK2
• NCBI Official Synonym Symbols: ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs
• NCBI Protein Information: activated CDC42 kinase 1; ACK-1; OTTHUMP00000208563; OTTHUMP00000208564; OTTHUMP00000208565; OTTHUMP00000208566; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; tyrosine kinase non-receptor protein 2
• UniProt Protein Name: Activated CDC42 kinase 1
• Protein Family: Activated CDC42 kinase
• UniProt Gene Name: TNK2
• UniProt Synonym Gene Names: ACK1
• UniProt Entry Name: ACK1_HUMAN

NCBI Description

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]

Uniprot Description

Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.11.1; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasmic vesicle membrane; clathrin-coated vesicle; dendrite; coated pit; extrinsic to internal side of plasma membrane; growth cone; adherens junction; cell soma; membrane; axon; cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; WW domain binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; GTPase inhibitor activity; ATP binding; epidermal growth factor receptor binding; hormone receptor binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cell migration; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; innate immune response; endocytosis; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation