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Western Blot (WB) (Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using MBS619735. (upper and middle) and Myc-Tag antibody (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing.)

Rabbit anti-Human ACK1 Polyclonal Antibody | anti-TNK2 antibody

ACK1, phosphorylated (Tyr284) (Activated CDC42 Kinase 1, ACK-1, ACK, FLJ44758, FLJ45547, p21cdc42Hs, Tyrosine Kinase Non-receptor Protein 2, TNK2)

Gene Names
TNK2; ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Purified by peptide affinity chromatography.
Synonyms
ACK1; Polyclonal Antibody; phosphorylated (Tyr284) (Activated CDC42 Kinase 1; ACK-1; ACK; FLJ44758; FLJ45547; p21cdc42Hs; Tyrosine Kinase Non-receptor Protein 2; TNK2); Anti -ACK1; anti-TNK2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes transfected levels of Ack1 only when phosphorylated at Tyr284. Species Sequence Homology: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-TNK2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr284 of human Ack1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using MBS619735. (upper and middle) and Myc-Tag antibody (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing.)

Western Blot (WB) (Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using MBS619735. (upper and middle) and Myc-Tag antibody (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing.)
Related Product Information for anti-TNK2 antibody
Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).
Product Categories/Family for anti-TNK2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
114,569 Da
NCBI Official Full Name
activated CDC42 kinase 1 isoform 2
NCBI Official Synonym Full Names
tyrosine kinase, non-receptor, 2
NCBI Official Symbol
TNK2
NCBI Official Synonym Symbols
ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs
NCBI Protein Information
activated CDC42 kinase 1; ACK-1; OTTHUMP00000208563; OTTHUMP00000208564; OTTHUMP00000208565; OTTHUMP00000208566; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; tyrosine kinase non-receptor protein 2
UniProt Protein Name
Activated CDC42 kinase 1
Protein Family
UniProt Gene Name
TNK2
UniProt Synonym Gene Names
ACK1
UniProt Entry Name
ACK1_HUMAN

NCBI Description

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]

Uniprot Description

Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: EC 2.7.11.1; Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasmic vesicle membrane; clathrin-coated vesicle; dendrite; coated pit; extrinsic to internal side of plasma membrane; growth cone; adherens junction; cell soma; membrane; axon; cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; WW domain binding; protein serine/threonine/tyrosine kinase activity; ATP binding; GTPase inhibitor activity; epidermal growth factor receptor binding; hormone receptor binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cell migration; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; innate immune response; endocytosis; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation

Research Articles on TNK2

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Product Notes

The TNK2 tnk2 (Catalog #AAA619735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACK1, phosphorylated (Tyr284) (Activated CDC42 Kinase 1, ACK-1, ACK, FLJ44758, FLJ45547, p21cdc42Hs, Tyrosine Kinase Non-receptor Protein 2, TNK2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the TNK2 tnk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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