Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat ACP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Rabbit anti-Rat Acid Phosphatase 1 (ACP1) Polyclonal Antibody | anti-ACP1 antibody
Polyclonal Antibody to Acid Phosphatase 1 (ACP1)
MGHHHHHHSGS-MAEVGSKSVL FVCLGNICRS PIAEAVFRKL VTDENVSDNW RIDSAATSTY EVGNPPDYRG QNCMKKHGIH MQHIARQITR EDFATFDYIL CMDESNLRDL NRKSNQVKNC KAKIELLGSY DPQKQLIIED PYYGNDSDFE VVYQQCLRCC KAFLEKTH
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat ACP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat ACP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded kidney tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: ACP1 knockout Hela cell lysate;;Predicted MW: 18kDa ;Observed MW: 17kDa;Primary Ab: 1ug/ml Rabbit Anti-Rat ACP1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: ACP1 knockout Hela cell lysate;;Predicted MW: 18kDa ;Observed MW: 17kDa;Primary Ab: 1ug/ml Rabbit Anti-Rat ACP1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
catalyzes the hydrolysis of p-nitrophenyl phosphate; may play a role in synaptic transmission [RGD, Feb 2006]
Uniprot Description
ACP1: a phosphotyrosine protein phosphatase. Functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. Hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. Genetically polymorphic with three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.
Protein type: Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2; EC 3.1.3.48; Motility/polarity/chemotaxis; Phosphatase (non-protein); Protein phosphatase, tyrosine (non-receptor)
Chromosomal Location of Human Ortholog: 6q16
Cellular Component: cytoplasm; cytoplasmic side of plasma membrane; cytosol; neuron projection; sarcolemma
Molecular Function: acid phosphatase activity; non-membrane spanning protein tyrosine phosphatase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity; SH3 domain binding
Biological Process: synaptic transmission
Research Articles on ACP1
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Product Notes
The ACP1 acp1 (Catalog #AAA2001656) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Acid Phosphatase 1 (ACP1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Acid Phosphatase 1 (ACP1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ACP1 acp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG S-MAEVGSKS VL FVCLGNICRS PIAEAVFRKL VTDENVSDNW RIDSAATSTY EVGNPPDYRG QNCMKKHGIH MQHIARQITR EDFATFDYIL CMDESNLRDL NRKSNQVKNC KAKIELLGSY DPQKQLIIED PYYGNDSDFE VVYQQCLRCC KAFLEKTH. It is sometimes possible for the material contained within the vial of "Acid Phosphatase 1 (ACP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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