Rabbit anti-Rat AcCoA Carboxylase 2 Polyclonal Antibody | anti-Acly antibody

AcCoA Carboxylase 2 (ACC-2, Acetyl Coenzyme A Carboxylase 2)

Cat. Num. AAA613942

• Gene Names: Acly; ACL; Clatp
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AcCoA Carboxylase 2; Polyclonal Antibody; AcCoA Carboxylase 2 (ACC-2; Acetyl Coenzyme A Carboxylase 2); Anti -AcCoA Carboxylase 2 (ACC-2; anti-Acly antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat AcCoA Carboxylase 2. No significant sequence homology is seen with ACC-1 or any other protein.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-Acly antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:10,000-1:100,000 using 50-100ng of A0225-03B per well; Western Blot: 1-10ug/ml using ECL.
• Immunogen: Synthetic peptide consisting of 20aa sequence near the N-terminus of rat ACC-2, KLH conjugated. Species Sequence Homology: mouse, 75%; human, 60%.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Acly antibody

In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and -2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.

Product Categories/Family for anti-Acly antibody

Antibodies; Abs to CoEnzyme A

NCBI and Uniprot Product Information

• NCBI GI #: 161760632
• NCBI GeneID: 24159
• NCBI Accession #: NP_001104565.1
• NCBI GenBank Nucleotide #: NM_001111095.1
• UniProt Accession #: P16638; Q497C7; Q8VIQ1
• Molecular Weight: 120,636 Da
• NCBI Official Full Name: ATP-citrate synthase isoform 2
• NCBI Official Synonym Full Names: ATP citrate lyase
• NCBI Official Symbol: Acly
• NCBI Official Synonym Symbols: ACL; Clatp
• NCBI Protein Information: ATP-citrate synthase; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
• UniProt Protein Name: ATP-citrate synthase
• Protein Family: ATP-citrate synthase
• UniProt Gene Name: Acly
• UniProt Entry Name: ACLY_RAT

NCBI Description

catalyzes the synthesis of cytosolic acetyl-CoA; plays a role in lipid and cholesterol metabolism [RGD, Feb 2006]

Uniprot Description

ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.3.8; Carbohydrate Metabolism - citrate (TCA) cycle; Lyase; Transferase

Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; plasma membrane; nucleus

Molecular Function: ATP citrate synthase activity; metal ion binding; lyase activity; cofactor binding; succinate-CoA ligase (ADP-forming) activity; ATP binding

Biological Process: acetyl-CoA metabolic process; lipid biosynthetic process; acetyl-CoA biosynthetic process; citrate metabolic process; cellular carbohydrate metabolic process; lipid metabolic process; fatty acid biosynthetic process