anti-Human ACACA Antibody | anti-ACACA antibody

ACACA Antibody

Cat. Num. AAA859160

• Gene Names: ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Protein G-Sepharose affinity purified.
• Synonyms: ACACA; ACACA Antibody; Rabbit polyclonal IgG to ACACA; anti-ACACA antibody

• Reactivity: Human
• Purity/Purification: Protein G-Sepharose affinity purified.
• Form/Format: Liquid
• Sequence Length: 2383

• Applicable Applications for anti-ACACA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:5000-1:20000; WB: 1:5000-1:10000
• Immunogen: Synthetic peptide corresponding to the C-terminus of human ACACA.
• Preservative: 0.02% Sodium Azide and 50% glycerol, 1% BSA, PBS, pH7.4.
• Cellular Localization: Cytoplasmic
• Preparation and Storage: Aliquot and store at-20 degree C. Avoid repeated freeze/thaw cycles.

Testing Data

(ACACA antibody at 1/10000 dilution interacts with A549 whole cell lysate 40ug/Lane. Predicted band size: 266KDa. Observed band size: 266KDa. Additional band size: 60KDa.)

Related Product Information for anti-ACACA antibody

In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA (precursor for long chain fatty acyl CoA, LCFA CoA) from acetyl CoA by Acetyl CoA carboxylase (ACC1) is the rate limiting step. Activities of ACC1 and 2 are regulated by their phosphorylation by 5' AMP activated protein kinase (AMPK).

Product Categories/Family for anti-ACACA antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 38679960
• NCBI GeneID: 31
• NCBI Accession #: NP_942131.1
• NCBI GenBank Nucleotide #: NM_198834.1
• UniProt Accession #: Q13085; Q6KEV6; Q6XDA8; Q7Z2G8; Q7Z561; Q7Z563; Q7Z564; Q86WB2; Q86WB3; B2RP68
• Molecular Weight: 269,999 Da
• NCBI Official Full Name: acetyl-CoA carboxylase 1 isoform 1
• NCBI Official Synonym Full Names: acetyl-CoA carboxylase alpha
• NCBI Official Symbol: ACACA
• NCBI Official Synonym Symbols: ACC; ACAC; ACC1; ACCA; ACACAD
• NCBI Protein Information: acetyl-CoA carboxylase 1
• UniProt Protein Name: Acetyl-CoA carboxylase 1
• UniProt Gene Name: ACACA
• UniProt Synonym Gene Names: ACAC; ACC1; ACCA; ACC1

NCBI Description

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.