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Western Blot (WB) (Western blot analysis of extracts of K562 cell lines, using ABO antibody.)

Rabbit ABO Polyclonal Antibody | anti-ABO antibody

ABO (Histo-blood Group ABO System Transferase, GTB, NAGAT, A3GALNT, A3GALT1) (AP)

Gene Names
ABO; GTB; NAGAT; A3GALNT; A3GALT1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
ABO; Polyclonal Antibody; ABO (Histo-blood Group ABO System Transferase; GTB; NAGAT; A3GALNT; A3GALT1) (AP); anti-ABO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes ABO. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-ABO antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:200
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human ABO.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of K562 cell lines, using ABO antibody.)

Western Blot (WB) (Western blot analysis of extracts of K562 cell lines, using ABO antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using ABO antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of MCF7 cell using ABO antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-ABO antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
28
Molecular Weight
354
NCBI Official Full Name
Histo-blood group ABO system transferase
NCBI Official Synonym Full Names
ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
NCBI Official Symbol
ABO
NCBI Official Synonym Symbols
GTB; NAGAT; A3GALNT; A3GALT1
NCBI Protein Information
histo-blood group ABO system transferase; ABO weak transfer; ABO A3 transferase; ABO glycosyltransferase; histo-blood group A transferase; histo-blood group B transferase; histo-blood group A2 transferase; B(A) alpha-1,3-galactosyltransferase; fucosylglyc
UniProt Protein Name
Histo-blood group ABO system transferase
UniProt Gene Name
ABO
UniProt Synonym Gene Names
A transferase; B transferase
UniProt Entry Name
BGAT_HUMAN

NCBI Description

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ABO: This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Belongs to the glycosyltransferase 6 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.37; Membrane protein, integral; EC 2.4.1.40; Transferase

Chromosomal Location of Human Ortholog: 9q34.2

Cellular Component: integral to membrane; extracellular region

Molecular Function: metal ion binding; glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity; fucosylgalactoside 3-alpha-galactosyltransferase activity

Biological Process: protein amino acid glycosylation

Disease: Blood Group, Abo System

Research Articles on ABO

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Product Notes

The ABO abo (Catalog #AAA6405134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABO (Histo-blood Group ABO System Transferase, GTB, NAGAT, A3GALNT, A3GALT1) (AP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ABO can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:200 WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ABO abo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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