Rabbit anti-Human ABL2 Polyclonal Antibody | anti-ABL2 antibody

ABL2, ID (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) (FITC)

Cat. Num. AAA6460896

• Gene Names: ABL2; ARG; ABLL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ABL2; Polyclonal Antibody; ID (Tyrosine-protein Kinase ABL2; Abelson Murine Leukemia Viral Oncogene Homolog 2; Abelson-related Gene Protein; Tyrosine Kinase ARG; ABLL; ARG) (FITC); anti-ABL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABL2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 1182

• Applicable Applications for anti-ABL2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the central region of human ABL2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Phospho-ABL-Y412 Antibody Pab in A2058 cell line lysate (35ug/lane). ABL (arrow) was detected using the purified Pab.)

Dot Blot (DB)

(Dot blot analysis of ABL-Y412 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-ABL2 antibody

Antibodies; Tyrosine Kinases

References

1. Cao, C., et al., Biochemistry 42(35):10348-10353 (2003). 2. Cao, C., et al., J. Biol. Chem. 278(32):29667-29675 (2003). 3. Kruh, G.D., et al., Proc. Natl. Acad. Sci. USA 87(15):5802-5806 (1990). 4. Kruh, G.D., et al., Science 234(4783):1545-1548 (1986). 5. Bianchi, C., et al., FEBS Lett. 527 (1-3), 216-222 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 6382062
• NCBI GeneID: 27
• NCBI Accession #: NP_009298
• NCBI GenBank Nucleotide #: NP_009298.1
• UniProt Accession #: P42684
• Molecular Weight: 128.343
• NCBI Official Full Name: tyrosine-protein kinase ABL2 isoform b
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: tyrosine-protein kinase ABL2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Abelson tyrosine-protein kinase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Oncoprotein; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extrinsic to internal side of plasma membrane; lamellipodium; dendritic spine; cytosol; actin cytoskeleton; phagocytic cup

Molecular Function: actin monomer binding; actin filament binding; protein binding; manganese ion binding; protein-tyrosine kinase activity; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; positive regulation of protein binding; alpha-beta T cell differentiation; cerebellum morphogenesis; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of apoptosis; Bergmann glial cell differentiation; elevation of cytosolic calcium ion concentration; regulation of endocytosis; visual learning; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; regulation of cell adhesion; epidermal growth factor receptor signaling pathway; actin filament bundle formation; cell migration; positive regulation of I-kappaB kinase/NF-kappaB cascade; dendrite morphogenesis; protein modification process; substrate-bound cell migration, cell extension; phagocytosis; regulation of cell proliferation; negative regulation of Rho protein signal transduction; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; neuron remodeling