Western Blot (WB)
(ABHD4 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ABHD4 antibody detected the ABHD4 protein (arrow).)
Rabbit anti-Mouse ABHD4 Polyclonal Antibody | anti-ABHD4 antibody
ABHD4, ID (ABHD4, Abhydrolase domain-containing protein 4, Alpha/beta-hydrolase 4, Lyso-N-acylphosphatidylethanolamine lipase)
Gene Names
ABHD4; ABH4
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ABHD4; Polyclonal Antibody; ID (ABHD4; Abhydrolase domain-containing protein 4; Alpha/beta-hydrolase 4; Lyso-N-acylphosphatidylethanolamine lipase); Anti -ABHD4; anti-ABHD4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-ABHD4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
ABHD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-127 amino acids from the Central region of human ABHD4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ABHD4 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ABHD4 antibody detected the ABHD4 protein (arrow).)
Western Blot (WB)
(ABHD4 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ABHD4 antibody detected the ABHD4 protein (arrow).)
Immunohistochemistry (IHC)
(ABHD4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ABHD4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ABHD4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ABHD4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ABHD4 antibody
Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (By similarity).
Product Categories/Family for anti-ABHD4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,794 Da
NCBI Official Full Name
abhydrolase domain-containing protein 4
NCBI Official Synonym Full Names
abhydrolase domain containing 4
NCBI Official Symbol
ABHD4
NCBI Official Synonym Symbols
ABH4
NCBI Protein Information
abhydrolase domain-containing protein 4; alpha/beta-hydrolase 4; lyso-N-acylphosphatidylethanolamine lipase
UniProt Protein Name
Abhydrolase domain-containing protein 4
Protein Family
UniProt Gene Name
ABHD4
UniProt Entry Name
ABHD4_HUMAN
Uniprot Description
ABHD4: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine. Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
Protein type: EC 3.1.1.-; Hydrolase
Chromosomal Location of Human Ortholog: 14q11.2
Molecular Function: hydrolase activity
Biological Process: lipid catabolic process
Research Articles on ABHD4
Similar Products
Product Notes
The ABHD4 abhd4 (Catalog #AAA6012527) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABHD4, ID (ABHD4, Abhydrolase domain-containing protein 4, Alpha/beta-hydrolase 4, Lyso-N-acylphosphatidylethanolamine lipase) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ABHD4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the ABHD4 abhd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABHD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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