Human Apolipoprotein A1 (APOA1) DIY ELISA Kit | APOA1 diy elisa kit

Human Apolipoprotein A1 (APOA1) ELISA Kit DIY Materials

Cat. Num. AAA2088612

• Gene Names: APOA1; apo(a)
• Reactivity: Human
• Synonyms: Apolipoprotein A1 (APOA1); Human Apolipoprotein A1 (APOA1) ELISA Kit DIY Materials; Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; APOA1 diy elisa kit

• Reactivity: Human
• Specificity: The Antibody Pairs in the kit have high sensitivity and excellent specificity for detection of APOA1.
• Sequence Length: 67

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Sandwich
• Detection Range: 31.2-2,000ng/mL
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA1 diy elisa kit

Intended Uses: This 'Do it Yourself (DIY)' assay kit contains materials for developing your own immunoassays. This may include the development of sandwich ELISA to measure APOA1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids in vitro. This kit contains sufficient materials for preparation of at least ten 96-well plates. Sample Preparation, Assay Protocol and Detection Range for Reference are provided as suggestions only. Researchers should optimize the use of kit materials and protocols for their own model system and determine if the kit is suitable for their samples and immunoassay.

Product Categories/Family for APOA1 diy elisa kit

Metabolic pathway; Cardiovascular biology; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 4960066
• NCBI GeneID: 335
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A1, partial
• NCBI Official Synonym Full Names: apolipoprotein A1
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: apo(a)
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell development/differentiation; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: chylomicron; cytoplasmic vesicle; cytosol; early endosome; endocytic vesicle; endoplasmic reticulum lumen; extracellular region; extracellular space; plasma membrane

Molecular Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; identical protein binding; phosphatidylcholine binding; phospholipid binding; protein binding

Biological Process: cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; G-protein coupled receptor protein signaling pathway; integrin-mediated signaling pathway; lipoprotein biosynthetic process; lipoprotein metabolic process; negative chemotaxis; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; neurite regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phospholipid efflux; phospholipid homeostasis; platelet degranulation; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; protein amino acid oxidation; protein stabilization; receptor-mediated endocytosis; regulation of Cdc42 protein signal transduction; regulation of cholesterol absorption; retinoid metabolic process; reverse cholesterol transport; transforming growth factor beta receptor signaling pathway; transmembrane transport; triacylglycerol catabolic process; vitamin transport

Disease: Amyloidosis, Familial Visceral; Hypoalphalipoproteinemia, Primary