Rabbit ABCG1 Polyclonal Antibody | anti-ABCG1 antibody

Anti-ABCG1 antibody

Cat. Num. AAA176215

• Gene Names: ABCG1; ABC8; WHITE1
• Reactivity: Reacts with: Human; Predicted to work with: Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: ABCG1; Polyclonal Antibody; Anti-ABCG1 antibody; ATP-binding cassette sub-family G member 1; ATP-binding cassette; sub-family G(WHITE); member 1; ABC transporter 8 antibody; Abc8 antibody; ABCG1 antibody; ABCG1_HUMAN antibody; ATP-binding cassette sub family G member 1 antibody; ATP-binding cassette sub-family G member 1 antibody; ATP-binding cassette transporter 8 antibody; ATP-binding cassette transporter member 1 of subfamily G antibody; sub family G WHITE member 1 antibodyhomolog of Drosophila white antibody; MGC34313 antibody; White protein homolog antibody; White protein homolog ATP binding cassette transporter 8 antibody; WHITE1 antibody; wht1 antibody; anti-ABCG1 antibody

• Host: Rabbit
• Reactivity: Reacts with: Human; Predicted to work with: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 678

• Applicable Applications for anti-ABCG1 antibody: Western Blot (WB)
• Application Notes: Western Blot: ; Concentration: 0.1-0.5ug/ml; Tested Species: Hu; Predicted Species: Ms, Rat; Antigen Retrieval: -; ; WB: The detection limit for ABCG1 is approximately 5ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results.; Predicted Species: Specices predicted to be fit for the product based on sequence similarities.; Other applications have not been tested. Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ABCG1 (627-647aa ETCHFQKSEAILRELDVENAK), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-ABCG1 antibody, MBS176215, Western blottingAll lanes: Anti ABCG1 (MBS176215) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 75KDObserved bind size: 75KD)

Related Product Information for anti-ABCG1 antibody

Description: Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 1(ABCG1) detection. Tested with WB in Human, Mouse, Rat.
Background: ABCG1(Atp-binding cassette, subfamily g, member 1) also known as ABC8, ABC TRANSPORTER 8 or WHITE, DROSOPHILA, HOMOLOG OF, is a protein that in humans is encoded by the ABCG1 gene. ABCG1 is a regulator of macrophage cholesterol and phospholipid transport. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters.The ABCG1 gene is mapped on 21q22.3. This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. ABCG1 is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Inhibition of ABCG1 protein expression using an antisense strategy resulted in reduced HDL3-dependent efflux of cholesterol and choline-phospholipids. In macrophages, silencing of GPS2 by RNA interference reduced ABCG1 expression and diminished ABCG1-mediated cholesterol efflux.

NCBI and Uniprot Product Information

• NCBI GI #: 17433715
• NCBI GeneID: 9619
• UniProt Accession #: P45844; Q86SU8; Q96L76; Q9BXK6; Q9BXK7; Q9BXK8; Q9BXK9; Q9BXL0; Q9BXL1; Q9BXL2; Q9BXL3; Q9BXL4
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 1
• NCBI Official Symbol: ABCG1
• NCBI Official Synonym Symbols: ABC8; WHITE1
• NCBI Protein Information: ATP-binding cassette sub-family G member 1; ABC transporter 8; homolog of Drosophila white; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; white protein homolog (ATP-binding cassette transporter 8)
• UniProt Protein Name: ATP-binding cassette sub-family G member 1
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG1
• UniProt Synonym Gene Names: ABC8; WHT1
• UniProt Entry Name: ABCG1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; recycling endosome; intracellular membrane-bound organelle; mitochondrion; integral to plasma membrane; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; phospholipid transporter activity; protein heterodimerization activity; cholesterol transporter activity; toxin transporter activity; cholesterol binding; phospholipid binding; sterol-transporting ATPase activity; ADP binding; ATP binding

Biological Process: cholesterol metabolic process; detection of hormone stimulus; response to high density lipoprotein stimulus; positive regulation of cholesterol biosynthetic process; intracellular cholesterol transport; cholesterol efflux; lipoprotein metabolic process; response to lipid; response to organic substance; amyloid precursor protein catabolic process; cholesterol homeostasis; reverse cholesterol transport; regulation of transcription, DNA-dependent; phospholipid efflux; transmembrane transport; phospholipid homeostasis