Rabbit anti-Human TIAM1 Polyclonal Antibody | anti-TIAM1 antibody

TIAM1, CT (TIAM1, T-lymphoma invasion and metastasis-inducing protein 1) (Azide free) (HRP)

Cat. Num. AAA6355496

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TIAM1; Polyclonal Antibody; CT (TIAM1; T-lymphoma invasion and metastasis-inducing protein 1) (Azide free) (HRP); T-lymphoma invasion and metastasis-inducing protein 1; anti-TIAM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TIAM1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TIAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1478-1506 amino acids from the C-terminal region of human TIAM1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TIAM1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the TIAM1 antibody detected the TIAM1 protein (arrow).)

Related Product Information for anti-TIAM1 antibody

Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA.

Product Categories/Family for anti-TIAM1 antibody

Antibodies; Cancer Markers

References

Yang, W., et al. Jpn. J. Clin. Oncol. 40(11):1053-1059(2010). Moriarty, C.H., et al. J. Biol. Chem. 285(27):20541-20546(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Rajagopal, S., et al. J. Biol. Chem. 285(23):18060-18071(2010). Shepherd, T.R., et al. J. Mol. Biol. 398(5):730-746(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 115583670
• NCBI GeneID: 7074
• NCBI Accession #: NP_003244.2
• NCBI GenBank Nucleotide #: NM_003253.2
• UniProt Accession #: Q13009; Q17RT7; B7ZLR6; F5GZ53
• Molecular Weight: 170,580 Da
• NCBI Official Full Name: T-lymphoma invasion and metastasis-inducing protein 1
• NCBI Official Synonym Full Names: T-cell lymphoma invasion and metastasis 1
• NCBI Official Symbol: TIAM1
• NCBI Protein Information: T-lymphoma invasion and metastasis-inducing protein 1
• UniProt Protein Name: T-lymphoma invasion and metastasis-inducing protein 1
• Protein Family: T-lymphoma invasion and metastasis-inducing protein
• UniProt Gene Name: TIAM1
• UniProt Synonym Gene Names: TIAM-1

Uniprot Description

TIAM1: modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP- dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA. Regulates migration, cell-matrix and cell-cell adhesion by modulating the actin cytoskeleton through the GTPase, Rac1. Binding to Rac1 and the Arp2/3 complex may drive actin polymerization at specific sites, thus inducing dynamic morphological changes.

Protein type: GEFs; GEFs, Rac/Rho; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cytosol; extrinsic to internal side of plasma membrane; intercellular junction; plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; kinase binding; protein binding; Rac guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: cell migration; cell-matrix adhesion; ephrin receptor signaling pathway; positive regulation of apoptosis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of protein binding; Rac protein signal transduction; regulation of small GTPase mediated signal transduction