Rabbit anti-Human, Rat AANAT Polyclonal Antibody | anti-AANAT antibody

AANAT Polyclonal Antibody

Cat. Num. AAA3006867

• Gene Names: AANAT; DSPS; SNAT
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AANAT; Polyclonal Antibody; AANAT Polyclonal Antibody; Serotonin N-acetyltransferase; Serotonin acetylase; Aralkylamine N-acetyltransferase; AA-NAT; SNAT; anti-AANAT antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AANAT polyclonal antibody detects endogenous levels of AANAT protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 207

• Applicable Applications for anti-AANAT antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human AANAT
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AANAT polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:PC12 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of AANAT pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic strong positive staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of AANAT pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic strong positive staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-AANAT antibody

AANAT (Aralkylamine N-acetyltransferase), also called Serotonin N-acetyltransferase (SNAT) or Serotonin acetylase, is a member of the acetyltransferase superfamily. It is almost exclusively expressed in the pineal gland and the retina. AANAT activity is high at night and low during the day due to the 10 to 100-fold increase in the amount of active AANAT at night. Retinal exposure to light causes cAMP levels to decrease in photoreceptor cells and, as a result, AANAT is targeted for degradation by proteasomal proteolysis. AANAT plays an important role as the rate limiting enzyme in melatonin synthesis. It is responsible for catalyzing the N-acetylation of serotonin to N-acetylserotonin, which is then converted to melatonin by hydroxyindole-O-methyltransferase. Melatonin is an important hormone that is involved in many physiological processes including immune function, seasonal reproduction, retinal physiology and circadian entrainment.

NCBI and Uniprot Product Information

• NCBI GI #: 116497189
• NCBI GeneID: 15
• UniProt Accession #: Q16613
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: Arylalkylamine N-acetyltransferase
• NCBI Official Synonym Full Names: aralkylamine N-acetyltransferase
• NCBI Official Symbol: AANAT
• NCBI Official Synonym Symbols: DSPS; SNAT
• NCBI Protein Information: serotonin N-acetyltransferase
• UniProt Protein Name: Serotonin N-acetyltransferase
• Protein Family: Serotonin N-acetyltransferase
• UniProt Gene Name: AANAT
• UniProt Synonym Gene Names: SNAT; Serotonin acetylase; AA-NAT
• UniProt Entry Name: SNAT_HUMAN

NCBI Description

The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]