Human PAI-1 / Serpin E ELISA Kit | PAI-1 elisa kit

PAI-1 / Serpin E Human ELISA Kit

Cat. Num. AAA355379

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Human
• Applications: ELISA
• Synonyms: PAI-1 / Serpin E; PAI-1 / Serpin E Human ELISA Kit; Human PAI-1 / Serpin E; PAI-1 elisa kit

• Reactivity: Human

• Applicable Applications for PAI-1 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of PAI-1 in plasma, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAI-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PAI-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PAI-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PAI-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PAI-1 can be calculated.

Background: Plasminogen activator inhibitor-1 (PAI-1) also known as endothelial plasminogen activator inhibitor or serpin E1 is a protein that in humans is encoded by the SERPINE1 gene. This gene is approximately 12.2 kb long and contains 9 exons, and located on the seventh chromosome (7q21.3-q22). PAI-1 is a member of the serine protease inhibitor family that inhibits tissue-type plasminogen activator and urokinase-type plasminogen activator. PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions.

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.4
• UniProt Accession #: P05121; B7Z4S0; F8WD53
• Molecular Weight: 45,060 Da
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN

NCBI Description

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. Ref.18

Subunit structure: Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Ref.15 Ref.17

Subcellular location: Secreted.

Tissue specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Post-translational modification: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.

Involvement in disease: Plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]: A hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

Sequence similarities: Belongs to the serpin family.