Rabbit AAK1 Polyclonal Antibody | anti-AAK1 antibody

AAK1 Antibody

Cat. Num. AAA7115881

• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: AAK1; Polyclonal Antibody; AAK1 Antibody; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1; anti-AAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; In Phosphate Buffered Saline, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.

• Applicable Applications for anti-AAK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:300; IF: 1:200-1:1000; ELISA: 1:20000
• Immunogen: Synthesized peptide derived from the Internal region of human AAK1.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot analysis of COS7 cells using AAK1 Polyclonal Antibody)

Product Categories/Family for anti-AAK1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 148277037
• NCBI GeneID: 22848
• NCBI Accession #: NP_055726.3
• NCBI GenBank Nucleotide #: NM_014911.3
• UniProt Accession #: Q2M2I8; Q4ZFZ3; Q53RX6; Q9UPV4
• Molecular Weight: 103,885 Da
• NCBI Official Full Name: AP2-associated protein kinase 1
• NCBI Official Synonym Full Names: AP2 associated kinase 1
• NCBI Official Symbol: AAK1
• NCBI Protein Information: AP2-associated protein kinase 1; adaptor-associated kinase 1
• UniProt Protein Name: AP2-associated protein kinase 1
• Protein Family: AP2-associated protein kinase
• UniProt Gene Name: AAK1
• UniProt Synonym Gene Names: KIAA1048
• UniProt Entry Name: AAK1_HUMAN

NCBI Description

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

Protein type: Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; NAK family

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: extrinsic to plasma membrane; clathrin-coated vesicle; leading edge; terminal button; coated pit

Molecular Function: protein serine/threonine kinase activity; protein binding; Notch binding; ATP binding

Biological Process: regulation of protein localization; protein stabilization; protein amino acid autophosphorylation; endocytosis; protein amino acid phosphorylation; positive regulation of Notch signaling pathway