CHI3L1 / YKL40 / gp39 recombinant protein
Recombinant Mouse CHI3L1 / YKL40 / gp39 Protein (His tag)
Gene Names
Chil1; Gp39; Brp39; Chi3l1; AW208766
Purity
> 97 % as determined by SDS-PAGE
Synonyms
CHI3L1 / YKL40 / gp39; Recombinant Mouse CHI3L1 / YKL40 / gp39 Protein (His tag); AW208766; Brp39; Gp39; CHI3L1 / YKL40 / gp39 recombinant protein
Host
Human Cells
Purity/Purification
> 97 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
389
Application Notes
The secreted recombinant mouse CHI3L1 comprises 371 amino acids and has a calculated molecular mass of 42.3 kDa. As a result of glycosylation, the apparent molecular mass of rmCHI3L1 is approximately 50 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Tyr 371
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
Related Product Information for CHI3L1 / YKL40 / gp39 recombinant protein
Background: Chitinase-3-like protein 1 (CHI3L1) is a secreted heparin-binding glycoprotein whose expression is associated with vascular smooth muscle cell migration. CHI3L1 is expressed at high levels in postconfluent nodular VSMC cultures and at low levels in subconfluent proliferating cultures. CHI3L1 is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyto / macrophage markers, its expression is absent in monocytes and strong induced during late stages of human macrophage differentiation. Elevated levels of CHI3L1 are associated with disorders exhibiting increased connective tissue turnover, such as rheum atoid, arthritis, osteoarthritis, scleroderma, and cirrhosis of liver, but is produced in cartilage from old donors or patiens with osteoarthritis. CHI3L1 is abnormally expressed in the hippocampus of subjects with schizophrenia and may be involved in the cellular response to various environmental events that are reported to increase the risk of schizophrenia.
Description: A DNA sequence encoding the extracellular domain of mouse CHI3L1 (Q61362) (Met 1-Ala 381) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the extracellular domain of mouse CHI3L1 (Q61362) (Met 1-Ala 381) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,805 Da
NCBI Official Full Name
chitinase-3-like protein 1
NCBI Official Synonym Full Names
chitinase-like 1
NCBI Official Symbol
Chil1
NCBI Official Synonym Symbols
Gp39; Brp39; Chi3l1; AW208766
NCBI Protein Information
chitinase-3-like protein 1
UniProt Protein Name
Chitinase-3-like protein 1
UniProt Gene Name
Chi3l1
UniProt Synonym Gene Names
Brp39; Chil1; CGP-39; GP-39
Uniprot Description
CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family.
Protein type: Cell adhesion; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1 E4|1 58.15 cM
Cellular Component: cytoplasm; endoplasmic reticulum; extracellular region; extracellular space
Molecular Function: chitin binding
Biological Process: activation of NF-kappaB-inducing kinase; lung development; positive regulation of angiogenesis; positive regulation of protein kinase B signaling cascade
