CHI3L1 / YKL40 / gp39 recombinant protein

Recombinant Mouse CHI3L1 / YKL40 / gp39 Protein (His tag)

Cat. Num. AAA2546736

• Gene Names: Chil1; Gp39; Brp39; Chi3l1; AW208766
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: CHI3L1 / YKL40 / gp39; Recombinant Mouse CHI3L1 / YKL40 / gp39 Protein (His tag); AW208766; Brp39; Gp39; CHI3L1 / YKL40 / gp39 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 389

• Application Notes: The secreted recombinant mouse CHI3L1 comprises 371 amino acids and has a calculated molecular mass of 42.3 kDa. As a result of glycosylation, the apparent molecular mass of rmCHI3L1 is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Tyr 371
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CHI3L1 / YKL40 / gp39 recombinant protein

Background: Chitinase-3-like protein 1 (CHI3L1) is a secreted heparin-binding glycoprotein whose expression is associated with vascular smooth muscle cell migration. CHI3L1 is expressed at high levels in postconfluent nodular VSMC cultures and at low levels in subconfluent proliferating cultures. CHI3L1 is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyto / macrophage markers, its expression is absent in monocytes and strong induced during late stages of human macrophage differentiation. Elevated levels of CHI3L1 are associated with disorders exhibiting increased connective tissue turnover, such as rheum atoid, arthritis, osteoarthritis, scleroderma, and cirrhosis of liver, but is produced in cartilage from old donors or patiens with osteoarthritis. CHI3L1 is abnormally expressed in the hippocampus of subjects with schizophrenia and may be involved in the cellular response to various environmental events that are reported to increase the risk of schizophrenia.

Description: A DNA sequence encoding the extracellular domain of mouse CHI3L1 (Q61362) (Met 1-Ala 381) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 224809461
• NCBI GeneID: 12654
• NCBI Accession #: NP_031721.2
• NCBI GenBank Nucleotide #: NM_007695.3
• UniProt Accession #: Q61362; Q3U291; Q4FJW9; Q8BKL8; Q99J84; B0FEU7; D3Z2P2
• Molecular Weight: 42,805 Da
• NCBI Official Full Name: chitinase-3-like protein 1
• NCBI Official Synonym Full Names: chitinase-like 1
• NCBI Official Symbol: Chil1
• NCBI Official Synonym Symbols: Gp39; Brp39; Chi3l1; AW208766
• NCBI Protein Information: chitinase-3-like protein 1
• UniProt Protein Name: Chitinase-3-like protein 1
• UniProt Gene Name: Chi3l1
• UniProt Synonym Gene Names: Brp39; Chil1; CGP-39; GP-39

Uniprot Description

CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family.

Protein type: Cell adhesion; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1 E4|1 58.15 cM

Cellular Component: cytoplasm; endoplasmic reticulum; extracellular region; extracellular space

Molecular Function: chitin binding

Biological Process: activation of NF-kappaB-inducing kinase; lung development; positive regulation of angiogenesis; positive regulation of protein kinase B signaling cascade