Typical Testing Data/Standard Curve (for reference only)
Rat nicotinamide phosphoribosyltransferase ELISA Kit | NAMPT elisa kit
Rat visfatin ELISA Kit
Gene Names
Nampt; Pbef; Pbef1
Reactivity
Rat
Synonyms
nicotinamide phosphoribosyltransferase; Rat visfatin ELISA Kit; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF; PBEF1; VF; VISFATIN; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; NAMPT elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat NAMPT. No significant cross-reactivity or interference between Rat NAMPT and analogues was observed.
Sequence Length
491
Samples
Serum, plasma, tissue homogenates
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
0.78 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Alkaloid biosynthesis and metabolism
Protein Biological Process 3
Pyridine nucleotide biosynthesis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NAMPT elisa kit
Introduction: Visfatin, an adipokine that is highly enriched in the visceral fat, has increased expression level in plasma during the development of obesity, implying its important role in obesity. Visfatin (also known as PBEF and Nampt), a 52 kDa cytoplasmic protein composed of 473-amino acid, expresses primarily in bone marrow, muscle and liver tissue but can also be detected in placenta, lung, kidney and heart tissue. In addition to its role in obesity, visfatin also has other biological functions. Visfatin is a nicotinamide phosphoribosyltransferase (Nampt) enzyme that catalyzes first step in the biosynthesis of NAD from nicotinamide. Visfatin has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to visfatin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for visfatin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain visfatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of visfatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to visfatin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for visfatin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain visfatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of visfatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,438 Da
NCBI Official Full Name
nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
Nampt
NCBI Official Synonym Symbols
Pbef; Pbef1
NCBI Protein Information
nicotinamide phosphoribosyltransferase; visfatin; NAmPRTase; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1 homolog
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
Protein Family
UniProt Gene Name
Nampt
UniProt Synonym Gene Names
Pbef1; NAmPRTase; Nampt; PBEF
UniProt Entry Name
NAMPT_RAT
NCBI Description
has similarity to cytokines but is not secreted; localized to the nucleus and cytoplasm, changes in subcellular localization may be associated with the cell cycle [RGD, Feb 2006]
Uniprot Description
Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function
Catalytic activity: Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate.
Research Articles on NAMPT
Similar Products
Product Notes
The Rat NAMPT nampt (Catalog #AAA700290) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700290 ELISA Kit recognizes Rat NAMPT. It is sometimes possible for the material contained within the vial of "nicotinamide phosphoribosyltransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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