SDS-PAGE
(Gel: 8%SDS-PAGE, Lysate: 0.5 ug, Lane: Recombinant 6HIS Tagged protein , Primary antibody: MBS7126728(6His Antibody) at dilution 1/2000 (lane 1),1/5000 (lane 2),1/8000 (lane 3),1/10000 (lane 4), Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
Rabbit 6×His Tag Polyclonal Antibody
6×His Tag Antibody
Reactivity
HIS Tag
Applications
ELISA, Western Blot
Purity
Antigen Affinity Purification
Synonyms
6×His Tag; Polyclonal Antibody; 6×His Tag Antibody; HHHHHH Tag; anti-6×His Tag antibody
Host
Rabbit
Reactivity
HIS Tag
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol
Applicable Applications for anti-6×His Tag antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:5000-1:10000
WB: 1:2000-1:10000
WB: 1:2000-1:10000
Immunogen
Synthetic peptide: 18×His conjugated to KLH
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
SDS-PAGE
(Gel: 8%SDS-PAGE, Lysate: 0.5 ug, Lane: Recombinant 6HIS Tagged protein , Primary antibody: MBS7126728(6His Antibody) at dilution 1/2000 (lane 1),1/5000 (lane 2),1/8000 (lane 3),1/10000 (lane 4), Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
SDS-PAGE
(Gel: 8%SDS-PAGE, Lysate: 0.5 ug, Lane: Recombinant 6HIS Tagged protein , Primary antibody: MBS7126728(6His Antibody) at dilution 1/2000 (lane 1),1/5000 (lane 2),1/8000 (lane 3),1/10000 (lane 4), Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
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Product Notes
The 6×His Tag (Catalog #AAA7126728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 6×His Tag Antibody reacts with HIS Tag and may cross-react with other species as described in the data sheet. AAA Biotech's 6×His Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:5000-1:10000 WB: 1:2000-1:10000. Researchers should empirically determine the suitability of the 6×His Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "6×His Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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