Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using GSK3beta (Phospho-Tyr216) antibody)
Rabbit GSK3 beta Polyclonal Antibody | anti-GSK3 beta antibody
GSK3 beta antibody
Gene Names
gsk3b.L; gsk3; gsk-3; gsk3b; xgsk-3; gsk3beta; gsk3-beta
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
GSK3 beta; Polyclonal Antibody; GSK3 beta antibody; Rabbit polyclonal GSK3 beta antibody; Polyclonal GSK3 beta antibody; Anti-GSK3 beta antibody; Phospho Tyr216 antibody; GSK beta 3; Glycogen synthase kinase 3 beta antibody; GSK beta-3; GSk3beta antibody; GSK beta-3 antibody; Phospho Ser9 antibody; GSK beta 3 antibody; anti-GSK3 beta antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
420
Applicable Applications for anti-GSK3 beta antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunogen
GSK3 beta antibody was raised in Rabbit using the peptide sequence around phosphorylation site of tyrosine 216 (V-S-Y(p)-I-C) derived from Human GSK3B as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using GSK3beta (Phospho-Tyr216) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using GSK3beta (Phospho-Tyr216) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from 293 cells untreated or treated with PMAusing GSK3beta (Phospho-Tyr216) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from 293 cells untreated or treated with PMAusing GSK3beta (Phospho-Tyr216) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3beta (Phospho-Tyr216) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3beta (Phospho-Tyr216) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-GSK3 beta antibody
Glycogen synthase kinase 3 beta is a serine/threonine kinase involved in insulin, growth factor and Wnt signaling. Glycogen synthase kinase 3 beta (GSk3beta) is a molecular regulator of cell fate in the central nervous system and a target of the phosphatidylinositol 3-kinase (PI3k) pathway. It is an enzyme with a diverse number of actions in intracellular signaling systems, regulating neuronal plasticity, gene expression and cell survival.
Product Categories/Family for anti-GSK3 beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,903 Da
NCBI Official Full Name
glycogen synthase kinase-3 beta
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta L homeolog
NCBI Official Symbol
gsk3b.L
NCBI Official Synonym Symbols
gsk3; gsk-3; gsk3b; xgsk-3; gsk3beta; gsk3-beta
NCBI Protein Information
glycogen synthase kinase-3 beta
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
gsk3b
UniProt Synonym Gene Names
Xgsk-3; GSK-3 beta
Uniprot Description
Plays a role in the organization of the formation of the main body axis of developing embryo. Acts as an inhibitor of differentiation of primary neurons. Inhibits the ability of ectopically expressed NEUROD1 and other bHLH factors to promote early retinal cell differentiation. May participate in the Wnt signaling pathway. May regulate the circadian clock via phosphorylation of the major clock components.
Research Articles on GSK3 beta
1. Novel roles for Plk and GSK3 regulation of ADAM13 function in cranial neural crest cell migration.
Similar Products
Product Notes
The GSK3 beta gsk3b (Catalog #AAA5309611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GSK3 beta can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the GSK3 beta gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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