Rabbit anti-Rat Cytochrome P450 Polyclonal Antibody | anti-Por antibody

Cytochrome P450 Reductase Antibody: ATTO 488

Cat. Num. AAA805659

• Gene Names: Por; CYPOR
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Cytochrome P450; Polyclonal Antibody; Cytochrome P450 Reductase Antibody: ATTO 488; Cytochrome C Reductase; CCR; CPR; P450R; CYPOR; DKFZp686G04235; FLJ26468; NADPH Cytochrome; P450 Reductase; NADPH dependent cytochrome P450 reductase; NADPH-cytochrome P450 reductase; POR; anti-Por antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 678

• Applicable Applications for anti-Por antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: 1:1000 (WB)
• Conjugate: ATTO 488
• Storage Buffer: PBS pH 7.4, 0.09% azide, 50% glycerol
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human, Mouse, Rat Human, Mouse and Rat Lysates showing detection of ~ 75 kDa Cytochrome P450 Reductase protein using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Lane 1: MW ladder. Lane 2: Human lysate,  A431. Lane 3: Mouse lung lysate.  Lane 4: Rat lysate, Rat Brain Membrane (RBM). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP antibody at 1:2000 for 1 hour at RT. Color Development: TMB solution for 11 min at RT. Predicted/Observed Size: ~ 75 kDa. Other Band(s): ~30 -250 in Rat lysate only.)

Related Product Information for anti-Por antibody

Background Info: Detects a ~77kDa protein corresponding to the molecular weight of native rat cytochrome P450 reductase

Scientific Background: Cytochrome P450 Reductase is an enyme that is required for the transfer of electrons from NADPH to cytochrome P450 in microsomes. It has also been found to transfer electrons to Heme oxygenase and cytochrome B5. Cytochrome P450 Reductase is found localized to the endoplasmic reticulum membrane, tethered by its hydrophobic N-terminal tail. Mutations in the gene encoding Cytochrome P450 Reductase (POR) have been found to cause congenital adrenal hyperplasia, Antley-Bixler syndrome, amenorrhea and disordered steroidogenesis.

Product Categories/Family for anti-Por antibody

Heat Shock

References

1. Pandey A.V. and Flück C.E. (2013). Pharmacology & therapeutics 138 (2): 229-54. 2. Jensen K. and Møller BL (2010). Phytochemistry 71 (2-3): 132-41. 3. Flück C.E. et al. (2004). Nature Genetics 36 (3): 228-230.

NCBI and Uniprot Product Information

• NCBI GI #: 13928780
• NCBI GeneID: 29441
• NCBI Accession #: NP_113764.1
• NCBI GenBank Nucleotide #: NM_031576.1
• UniProt Accession #: P00388
• Molecular Weight: 76,963 Da
• NCBI Official Full Name: NADPH--cytochrome P450 reductase
• NCBI Official Synonym Full Names: P450 (cytochrome) oxidoreductase
• NCBI Official Symbol: Por
• NCBI Official Synonym Symbols: CYPOR
• NCBI Protein Information: NADPH--cytochrome P450 reductase; CPR; P450R
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• Protein Family: Cytochrome
• UniProt Gene Name: Por
• UniProt Synonym Gene Names: CPR; P450R
• UniProt Entry Name: NCPR_RAT

NCBI Description

plays a role in electron transport [RGD, Feb 2006]

Uniprot Description

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.

Protein type: Membrane protein, peripheral; Hydrolase; Oxidoreductase; Mitochondrial; Endoplasmic reticulum; Apoptosis; EC 1.6.2.4

Cellular Component: endoplasmic reticulum membrane; mitochondrion; intracellular membrane-bound organelle; membrane; cytosol

Molecular Function: FAD binding; hydrolase activity; cytochrome-b5 reductase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; enzyme binding; electron carrier activity; NADPH-hemoprotein reductase activity; FMN binding; nitric oxide dioxygenase activity; iron ion binding; NADP binding; iron-cytochrome-c reductase activity

Biological Process: response to drug; nitric oxide catabolic process; internal peptidyl-lysine acetylation; positive regulation of monooxygenase activity; positive regulation of cholesterol biosynthetic process; negative regulation of caspase activity; negative regulation of lipase activity; nitrate catabolic process; positive regulation of chondrocyte differentiation; flavonoid metabolic process; positive regulation of smoothened signaling pathway; carnitine metabolic process; fatty acid oxidation; response to nutrient; negative regulation of apoptosis