Western Blot (WB)
(Western blot analysis of 5-HT4 expression in U87MG (A), rat heart (B) whole cell lysates.)
Rabbit 5-HT4 Polyclonal Antibody | anti-5-HT4 antibody
Anti-5-HT4 Antibody
IF/ICC: 1:100-1:500
Western Blot (WB)
(Western blot analysis of 5-HT4 expression in U87MG (A), rat heart (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of 5-HT4 expression in U87MG (A), rat heart (B) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of 5-HT4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of 5-HT4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]
Uniprot Description
5-HT(4): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 5q31-q33
Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; endosome
Molecular Function: serotonin receptor activity
Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cell proliferation; regulation of appetite; serotonin receptor signaling pathway
Research Articles on 5-HT4
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Product Notes
The 5-HT4 htr4 (Catalog #AAA8305798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-5-HT4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 5-HT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the 5-HT4 htr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-HT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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