Flow Cytometry (FC/FACS)
(Staining of Yac-1 cell line with 0.125 ug of Rat IgG2b K Isotype Control (biotin) (open histogram) or 0.125 ug of RAE1 gamma antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Rat RAE1g Monoclonal Antibody | anti-RAE1g antibody
RAE1g antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
RAE1g; Monoclonal Antibody; RAE1g antibody (biotin); Monoclonal RAE1g; Anti-RAE1g; RAEg-1; RAE1gamma; RAE1-g; RAEg 1; RAE-1-g; anti-RAE1g antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
CX1
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-RAE1g antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 1 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Biological Significance
The CX1 monoclonal antibody reacts with the mouse retinoic acid early inducible gamma and weakly cross-reacts with the RAE-1 alpha and beta, but not with delta and epsilon. These 5 members of the RAE-1 family, along with another protein encoded by the minor histocompatibility gene complex called H60 have been identified as ligands for the mouse NKG2D molecule. These ligands are normally absent from adult tissue, however the presence of retinoic acid or certain disease conditions can induce up-regulation of this protein at the cell surface. RAE-1 alpha, -beta, and -gamma and H60 are expressed in BALB/c mice, but not in C57BL/6, whereas RAE-1delta, and -epsilon are expressed in C57BL/6 mice. It is reported that in NOD mice, both NKG2D and its ligand RAE-1 are expressed on the same NK cells, resulting in self-modulation of NKG2D expression and function.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of Yac-1 cell line with 0.125 ug of Rat IgG2b K Isotype Control (biotin) (open histogram) or 0.125 ug of RAE1 gamma antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of Yac-1 cell line with 0.125 ug of Rat IgG2b K Isotype Control (biotin) (open histogram) or 0.125 ug of RAE1 gamma antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Related Product Information for anti-RAE1g antibody
Rat monoclonal RAE1g antibody (biotin)
Product Categories/Family for anti-RAE1g antibody
Similar Products
Product Notes
The RAE1g (Catalog #AAA833946) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RAE1g can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 1 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the RAE1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAE1g, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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