Western Blot (WB)
(Sample Data: Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min) using 4EBP1 antibody (lanes 1 and 2) and anti-phospho-4EBP1 (Thr45) antibody (lanes 3 and 4).)
Rabbit 4EBP1 Polyclonal Antibody | anti-phospho-4EBP1 antibody
Anti-phospho-4EBP1 (Thr45)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity chromatography
Synonyms
4EBP1; Polyclonal Antibody; Anti-phospho-4EBP1 (Thr45); anti-phospho-4EBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the 4EBP1 protein phosphorylated at threonine 45
Purity/Purification
Affinity chromatography
Form/Format
PBS (pH7.4) 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-4EBP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr45
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min) using 4EBP1 antibody (lanes 1 and 2) and anti-phospho-4EBP1 (Thr45) antibody (lanes 3 and 4).)
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min) using 4EBP1 antibody (lanes 1 and 2) and anti-phospho-4EBP1 (Thr45) antibody (lanes 3 and 4).)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-4EBP1 (Thr45) antibody.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-4EBP1 (Thr45) antibody.)
Related Product Information for anti-phospho-4EBP1 antibody
The translational repressor protein 4EBP1 interacts directly with eukaryotic translation initiation factor 4E (EIF4E). This interaction inhibits the formation of the multi-subunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs thus preventing cap-dependent translation. Hyperphosphorylation of 4EBP1 disrupts its binding with EIF4E (1). Treating adipose cells with insulin leads to increased 4EBP1 phosphorylation (1). 4EBP1 is expressed in most tissues, with high levels detected in adipose tissue, pancreas and skeletal muscle (2). 4EBP1 is phosphorylated at several residues including Thr45 in response to the phorbol ester TPA.
Product Categories/Family for anti-phospho-4EBP1 antibody
References
1. Pause, A. et al: Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5-prime-cap function. Nature371: 762-767, 1994.
,br>2. Tsukiyama-Kohara, K. et al: Tissue distribution, genomic structure, and chromosome mapping of mouse and human eukaryotic initiation factor 4E-binding proteins 1 and 2. Genomics38: 353-363, 1996.
,br>2. Tsukiyama-Kohara, K. et al: Tissue distribution, genomic structure, and chromosome mapping of mouse and human eukaryotic initiation factor 4E-binding proteins 1 and 2. Genomics38: 353-363, 1996.
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Product Notes
The phospho-4EBP1 (Catalog #AAA515953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-4EBP1 (Thr45) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 4EBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the phospho-4EBP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4EBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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