Western Blot (WB)
(Western blot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.)
Mouse 4E-BP1/2/3 Monoclonal Antibody | anti-4E-BP1/2/3 antibody
4E-BP1/2/3 (Phospho-Thr 45) Antibody
IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
Western Blot (WB)
(Western blot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.)
Western Blot (WB)
(Western blot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.)
Western Blot (WB)
(Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A, D), untreated human 4E-BP1 transfected (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A, B, C) and 4E-BP1 (D, E, F).)
Western Blot (WB)
(Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A, D), untreated human 4E-BP1 transfected (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A, B, C) and 4E-BP1 (D, E, F).)
NCBI and Uniprot Product Information
NCBI Description
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]
Uniprot Description
ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.
Protein type: Membrane protein, integral; Vesicle; Transporter; Membrane protein, multi-pass; Transporter, ABC family
Chromosomal Location of Human Ortholog: 9q31.1
Cellular Component: Golgi apparatus; integral to plasma membrane; perinuclear region of cytoplasm; endocytic vesicle; plasma membrane; phagocytic vesicle; external side of plasma membrane; lipid raft
Molecular Function: apolipoprotein A-I receptor activity; ATPase activity; cholesterol binding; apolipoprotein binding; ATPase binding; apolipoprotein A-I binding; small GTPase binding; protein binding; syntaxin binding; phospholipid transporter activity; cholesterol transporter activity; phospholipid binding; anion transmembrane transporter activity; ATP binding; receptor binding
Biological Process: response to drug; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; lipoprotein metabolic process; intracellular cholesterol transport; cholesterol efflux; cellular lipid metabolic process; endosome transport; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; platelet dense granule organization and biogenesis; lysosome organization and biogenesis; response to low density lipoprotein stimulus; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; phagocytosis, engulfment; response to nutrient; phospholipid homeostasis; peptide secretion
Disease: Hypoalphalipoproteinemia, Primary; Tangier Disease; Hypercholesterolemia, Familial
Research Articles on 4E-BP1/2/3
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Product Notes
The 4E-BP1/2/3 abca1 (Catalog #AAA9430982) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 4E-BP1/2/3 (Phospho-Thr 45) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 4E-BP1/2/3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:100-1:1000 IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate). Researchers should empirically determine the suitability of the 4E-BP1/2/3 abca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4E-BP1/2/3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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