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Western Blot (WB) (Western blot analysis of Phospho-4E-BP1 (Thr45) in lysates of Jurkat Insulin 0.01U/ml 15', using Phospho-4E-BP1 (Thr45) Antibody(13223).)

Rabbit 4E-BP1 Polyclonal Antibody | anti-4E-BP1 antibody

4E-BP1 (Phospho-Thr45) Antibody

Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
4E-BP1; Polyclonal Antibody; 4E-BP1 (Phospho-Thr45) Antibody; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;; anti-4E-BP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-4E-BP1 (Phospho-Thr45) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr45
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (varies by lot)
Sequence Length
118
Applicable Applications for anti-4E-BP1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Phospho-4E-BP1 (Thr45) in lysates of Jurkat Insulin 0.01U/ml 15', using Phospho-4E-BP1 (Thr45) Antibody(13223).)

Western Blot (WB) (Western blot analysis of Phospho-4E-BP1 (Thr45) in lysates of Jurkat Insulin 0.01U/ml 15', using Phospho-4E-BP1 (Thr45) Antibody(13223).)
Product Categories/Family for anti-4E-BP1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Gene Name
EIF4EBP1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I

NCBI Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Uniprot Description

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Research Articles on 4E-BP1

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Product Notes

The 4E-BP1 eif4ebp1 (Catalog #AAA9429375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 4E-BP1 (Phospho-Thr45) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 4E-BP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the 4E-BP1 eif4ebp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4E-BP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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