Western Blot (WB)
(Western Blot: Sample: Recombinant HMGCR, Rat.)
Rabbit anti-Rat 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Polyclonal Antibody | anti-HMGCR antibody
3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant HMGCR, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HMGCR, Rat.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI Description
enzyme involved in mevalonate synthesis [RGD, Feb 2006]
Uniprot Description
HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.
Protein type: EC 1.1.1.34; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Oxidoreductase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis
Chromosomal Location of Human Ortholog: 2q12
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral component of membrane; intracellular membrane-bound organelle; peroxisomal membrane
Molecular Function: coenzyme binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; NADPH binding; protein homodimerization activity; protein phosphatase 2A binding
Biological Process: aging; cholesterol biosynthetic process; coenzyme A metabolic process; farnesyl diphosphate biosynthetic process, mevalonate pathway; isoprenoid biosynthetic process; myoblast differentiation; negative regulation of apoptosis; negative regulation of blood vessel diameter; negative regulation of MAP kinase activity; negative regulation of striated muscle cell apoptotic process; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of skeletal muscle tissue development; positive regulation of smooth muscle cell proliferation; positive regulation of stress-activated MAPK cascade; protein tetramerization; response to ethanol; response to nutrient; ubiquinone metabolic process; visual learning
Research Articles on HMGCR
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Product Notes
The HMGCR hmgcr (Catalog #AAA2032397) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HMGCR hmgcr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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