Western Blot (WB)
(Western blot analysis of 14-3-3 (Phospho-Ser186) Antibody expression in EGF treated HepG2 cells lysates..-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit 14-3-3 sigma Polyclonal Antibody | anti-14-3-3sigma antibody
Phospho-14-3-3 sigma (Ser186) Antibody
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (89%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (89%)
Tissue Specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Peptide ELISA: 1:20,000-1:40,000
Note: May be secreted by a non-classical secretory pathway.
Western Blot (WB)
(Western blot analysis of 14-3-3 (Phospho-Ser186) Antibody expression in EGF treated HepG2 cells lysates..-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of 14-3-3 (Phospho-Ser186) Antibody expression in EGF treated HepG2 cells lysates..-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
NCBI and Uniprot Product Information
Uniprot Description
14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; nucleus; cytosol
Molecular Function: protein kinase C inhibitor activity; protein domain specific binding; protein binding; phosphoprotein binding; protein kinase binding
Biological Process: positive regulation of protein export from nucleus; regulation of epidermal cell division; release of cytochrome c from mitochondria; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; regulation of cyclin-dependent protein kinase activity; negative regulation of caspase activity; signal transduction; positive regulation of cell growth
Research Articles on 14-3-3sigma
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Product Notes
The 14-3-3sigma sfn (Catalog #AAA9614451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-14-3-3 sigma (Ser186) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (89%) and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 sigma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:1000-3000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the 14-3-3sigma sfn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 sigma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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