Rabbit 14-3-3 gamma Polyclonal Antibody | anti-14-3-3 antibody

14-3-3 gamma (M81) Polyclonal Antibody

Cat. Num. AAA3002713

• Gene Names: YWHAG; EIEE56; PPP1R170; 14-3-3GAMMA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: 14-3-3 gamma; Polyclonal Antibody; 14-3-3 gamma (M81) Polyclonal Antibody; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1; YWHAG; KCIP 1; KCIP1; 1433G; anti-14-3-3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: 14-3-3 gamma (M81) polyclonal antibody detects endogenous levels of 14-3-3 gamma protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 247

• Applicable Applications for anti-14-3-3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human 14-3-3 gamma.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of 14-3-3 gamma (M81) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:SP2/0 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of 14-3-3 gamma (M81) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of 14-3-3 gamma (M81) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of 14-3-3 gamma (M81) pAb at 1:500 dillutionLane1:Hela whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug))

Related Product Information for anti-14-3-3 antibody

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

NCBI and Uniprot Product Information

• NCBI GI #: 21464101
• NCBI GeneID: 7532
• NCBI Accession #: NP_036611.2
• NCBI GenBank Nucleotide #: NP_036611.2
• UniProt Accession #: P61981
• Molecular Weight: ~ 28kDa
• NCBI Official Full Name: 14-3-3 protein gamma
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
• NCBI Official Symbol: YWHAG
• NCBI Official Synonym Symbols: EIEE56; PPP1R170; 14-3-3GAMMA
• NCBI Protein Information: 14-3-3 protein gamma
• UniProt Protein Name: 14-3-3 protein gamma
• UniProt Gene Name: YWHAG
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433G_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding

Biological Process: cellular response to insulin stimulus; apoptosis; regulation of neuron differentiation; organelle organization and biogenesis; regulation of signal transduction; regulation of synaptic plasticity; negative regulation of protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting