Testing Data
Rabbit 14-3-3 beta Polyclonal Antibody | anti-14-3-3 beta antibody
Rabbit anti 14-3-3 beta Polyclonal antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
The rabbit IgG is purified by Epitope- Affinity Purification.
Synonyms
14-3-3 beta; Polyclonal Antibody; Rabbit anti 14-3-3 beta Polyclonal antibody; Rabbit anti-14.3.3 beta; YWHAB; GW128; HS1; KCIP-1; anti-14-3-3 beta antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody recognizes ~28 kDa of human 14.3.3 beta. It does not react to the other isoforms. This antibody reacts with human, rat and mouse. The other species are not tested.
Purity/Purification
The rabbit IgG is purified by Epitope- Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-14-3-3 beta antibody
Western Blot, Immunoprecipitation, Immunohistochemistry - Paraffin
Application Notes
Western Blot: 1-2 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
Antigen Preparation
A synthetic peptide corresponding to N-term of human 14.3.3 beta protein.
Positive Control
3T3, Brain tissue
Cellular Location
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-14-3-3 beta antibody
The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14.3.3 beta is highly expressed in brain tissue. It is critical to cell transformation and mitotic signaling.
References
Zhi-jun Luo, et al. Identification of the 14.3.3 Domains Important for Self-association and Raf Binding. J. Bio. Chem 270 (40), 23681-23687, 1995
Similar Products
Product Notes
The 14-3-3 beta (Catalog #AAA462182) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti 14-3-3 beta Polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunoprecipitation, Immunohistochemistry - Paraffin. Western Blot: 1-2 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:100. Researchers should empirically determine the suitability of the 14-3-3 beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
