Pfu DNA Polymerase Recombinant Protein | PF0212 recombinant protein
Recombinant Pfu DNA Polymerase
Synonyms
Pfu DNA Polymerase; Recombinant Pfu DNA Polymerase; Pfu-DNA Polymerase Recombinant; DNA polymerase; EC 2.7.7.7; Pfu polymerase; Pfu-DNA Polymerase; PF0212 recombinant protein
Host
E Coli
Form/Format
50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol.
Sterile liquid formulation.
Sterile liquid formulation.
Sequence Length
775
Application Notes
1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3' overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75 degree C. 7. 95% active after 1-hour incubation at 98 degree C.
10X Buffer with MgSO4
200mM Tris-HCl (pH 8.8 at 25 degree C), 100mM (NH4)2SO4, 100mM KC1, 1% Triton X-100, 1mg/ml BSA, 20mM MgSO4.
PCR Protocol
Add the following components to a amplify 1kb DNA template: 5 ul 10x buffer with MgSO4. 4 ul 2.5mM dNTPs. 1 ul Primers mix (10 uM each). 0.2 ul Pfu-DNA Polymerase. and 38 ul Water. Amplify using the following cycling parameters: Heat Soak: 1 cycle at 94 degree C/4 min. Denaturation: 30 cycles at 94 degree C/30 sec. Annealing: 30 cycles at 58 degree C /30 sec. Extension: 30 cycles at 72 degree C /90 sec. Final: 1 cycle at 72 degree C /5 min.
Unit Defenition
1U of enzyme catalyzes the incorporation of 10nmol of dNTP into acid-insoluble product in 30 minutes at 75 degree C.
Preparation and Storage
Pfu DNA Polymerase although stable at 10 degree C for 5 days, should be stored below -18 degree C.Please prevent freeze-thaw cycles.
Related Product Information for PF0212 recombinant protein
Description: Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E Coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75 degree C, catalyzing the polymerization of nucleotides into duplex DNA in the 5 ´ to 3 ´ direction in the existence of magnesium. Pfu DNA Polymerase possesses 3 ´ to 5 ´ exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
Introduction: Pfu DNA polymeraseenzyme is found in the hyperthermophilic archaeonPyrococcus furiosus, where it functions in vivoto replicate the organism's DNA. In vitro, Pfu is used to swiftly amplify DNAin the Polymerase Chain Reaction, where the enzyme serves the central function of copying a new strand of DNA during each extension step. Pfu DNA polymerase has superior thermostability and 'proofreading' properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proof reading activity, meaning that it works its way along the DNA from the 5' endto the 3' endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts. As a result, Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.
Introduction: Pfu DNA polymeraseenzyme is found in the hyperthermophilic archaeonPyrococcus furiosus, where it functions in vivoto replicate the organism's DNA. In vitro, Pfu is used to swiftly amplify DNAin the Polymerase Chain Reaction, where the enzyme serves the central function of copying a new strand of DNA during each extension step. Pfu DNA polymerase has superior thermostability and 'proofreading' properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proof reading activity, meaning that it works its way along the DNA from the 5' endto the 3' endand corrects nucleotidemisin corporation errors. Pfu DNA polymerase-generated PCRfragments will have fewer errors than Taq-generated PCR inserts. As a result, Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.
Product Categories/Family for PF0212 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,113 Da
NCBI Official Full Name
DNA-directed DNA polymerase
NCBI Official Symbol
PF0212
NCBI Protein Information
DNA-directed DNA polymerase
UniProt Protein Name
DNA polymerase
UniProt Gene Name
pol
UniProt Entry Name
DPOL_PYRFU
Uniprot Description
In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.
Research Articles on PF0212
Similar Products
Product Notes
The PF0212 pol (Catalog #AAA142406) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. 1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3' overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75 degree C. 7. 95% active after 1-hour incubation at 98 degree C. Researchers should empirically determine the suitability of the PF0212 pol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pfu DNA Polymerase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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